3.4.22.B69: falcipain-2
This is an abbreviated version!
For detailed information about falcipain-2, go to the full flat file.
Word Map on EC 3.4.22.B69
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3.4.22.B69
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plasmodium
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falciparum
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antimalarial
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malaria
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hemoglobin
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falcipain-3
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antiplasmodial
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papain-family
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chloroquine-resistant
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hemoglobinases
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rhodesain
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prodomains
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plasmepsins
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hemozoin
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cruzain
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thiosemicarbazone
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chloroquine-sensitive
- 3.4.22.B69
- plasmodium
- falciparum
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antimalarial
- malaria
- hemoglobin
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falcipain-3
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antiplasmodial
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papain-family
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chloroquine-resistant
- hemoglobinases
- rhodesain
- prodomains
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plasmepsins
- hemozoin
- cruzain
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thiosemicarbazone
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chloroquine-sensitive
Reaction
The enzyme plays a key role in the hydrolysis of hemoglobin by the parasite Plasmodium falciparum. Preferred cleavage sites display arginine in P1, leucine in P2, and phenylalanine in P1' =
Synonyms
falcipain 2, falcipain-2, FP-2, Fp2, haemoglobinase
ECTree
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Substrates Products
Substrates Products on EC 3.4.22.B69 - falcipain-2
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REACTION DIAGRAM
Abz-MISLMKRPPGFSPFRSSRI-NH2 + H2O
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the peptide corresponds to the Met375 to Ile393 sequence of high molecular weight kininogen. The enzyme preferentially accommodates at the S1 subsite the positively charged residue Arg, followed by Gln. At the P2 position, the enzyme shows a clear preference for the hydrophobic aliphatic residue Leu over Phe. The subsite S3 of FP-2 shows a broad specificity with slight preference of Lys in the P3 position
the generated fragments are KRPPGFSPFR (Lys-bradykinin, 63%), RPPGFSPFR (bradykinin, 30%), and MKRPPGFSPFR (Met-Lys-bradykinin, 7%)
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benzyloxycarbonyl-Leu-Arg-7-amido-4-methylcoumarin + H2O
benzyloxycarbonyl-Leu-Arg + 7-amino-4-methylcoumarin
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benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin + H2O
benzyloxycarbonyl-Phe-Arg + 7-amino-4-methylcoumarin
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Cbz-Phe-Arg-7-amido-4-methylcoumarin + H2O
Cbz-Phe-Arg + 7-amino-4-methylcoumarin
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high molecular weight kininogen + H2O
Met-Lys-bradykinin + Lys-bradykinin + bradykinin + ?
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N-benzyloxycarbonyl-Leu-Arg 7-amido-4-methylcoumarin + H2O
N-benzyloxycarbonyl-Leu-Arg + 7-amino-4-methylcoumarin
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Z-Leu-Arg-7-amido-4-methylcoumarin + H2O
Z-Leu-Arg + 7-amino-4-methylcoumarin
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Z-Phe-Arg-7-amido-4-methylcoumarin + H2O
Z-Phe-Arg + 7-amino-4-methylcoumarin
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additional information
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the falcipain-2 prodomain efficiently inhibits falcipain-2, falcipain-2', berghepain-2, falcipain-3, cathepsin K, cathepsin L, cathepsin B, and cruzain, but it does not inhibit cathepsin C, pepsin, alpha-chymotrypsin, and collagenase
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D-Val-Leu-Arg + 7-amino-4-methylcoumarin
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D-Val-Leu-Arg-7-amido-4-methylcoumarin + H2O
D-Val-Leu-Arg + 7-amino-4-methylcoumarin
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