3.4.22.B68: EhCP4 proteinase

This is an abbreviated version, for detailed information about EhCP4 proteinase, go to the full flat file.

Reaction

unique preference for valine and isoleucine at P2 and broader specificities at the P1 and P3 positions, while proline at P4 is relatively selective =

Synonyms

CP4, cysteine proteinase 4

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.22 Cysteine endopeptidases
                3.4.22.B68 EhCP4 proteinase

Cloned

Cloned on EC 3.4.22.B68 - EhCP4 proteinase

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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli BL21 Condon Plus (DE3)-RIPL cells as as a fusion protein with bacterial thioredoxin A with the His tag at the amino terminus of the prodomain