3.4.22.B67: Ulp2 peptidase
This is an abbreviated version!
For detailed information about Ulp2 peptidase, go to the full flat file.
Word Map on EC 3.4.22.B67
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3.4.22.B67
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sumoylation
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checkpoint
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deconjugation
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sumo-1
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sumo-protein
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helicase
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sumo-specific
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hydroxyurea
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restart
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nuclear-localization
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poly-sumo
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dna-damaging
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nucleolar
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ubiquitin-related
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spindle
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euploidy
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polysumoylation
- 3.4.22.B67
-
sumoylation
-
checkpoint
-
deconjugation
- sumo-1
-
sumo-protein
- helicase
-
sumo-specific
- hydroxyurea
-
restart
-
nuclear-localization
-
poly-sumo
-
dna-damaging
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nucleolar
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ubiquitin-related
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spindle
-
euploidy
-
polysumoylation
Reaction
Ulp2 hydrolyses the SUMO-SUMO linkage in poly-SUMO chains. It catalyzes desumoylation of SUMO-modified-Ndc10 kinetochore protein, SUMO-modified-Bir1 kinetochore protein or SUMO-modified-Cep3 kinetochore protein. =
Synonyms
At1g09730, at4g33620, probable ubiquitin-like-specific protease 2A, probable ubiquitin-like-specific protease 2B, Smt3-specific protease, SMT4, Spf1, SPF2, SUMO de-conjugating/chain-editing enzyme Ulp2p, SUMO isopeptidase Ulp2, SUMO protease, SUMO(Smt3)-specific protease, SUMO-specific protease, Ulp2, ULP2 SUMO isopeptidase, Ulp2 SUMO protease, Ulp2A, Ulp2B, Ulp2p
ECTree
3.4.22.B67 Ulp2 peptidaseAdvanced search results
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results (Substrates Products
Substrates Products on EC 3.4.22.B67 - Ulp2 peptidase
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SUBSTRATE 
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
polySUMO-protein conjugate + H2O
SUMO + SUMO-SUMO + SUMO-SUMO-SUMO + truncated polySUMO-protein conjugate
Smt3-precursor + H2O
SMT3 + peptide
Ulp2 is able to process the Smt3 precursor in vivo, albeit inefficiently relative to Ulp1
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?
SUMO-modified-Bir1 kinetochore protein + H2O
SUMO + Bir1 kinetochore protein
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?
SUMO-modified-Cep3 kinetochore protein + H2O
SUMO + Cep3 kinetochore protein
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?
SUMO-modified-Ndc10 kinetochore protein + H2O
SUMO + Ndc10 kinetochore protein
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?
sumoylated human eIF4GI + H2O
?
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translation initiation factor and scaffold protein eIF4G is SUMOylated and associates with the SUMO protease Ulp2, partial co-localisation of recombinant human eIF4G and SUMO in stressed HeLa cells
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?
tetra-SUMO + H2O
SUMO + ?
55% degradation within 60 min
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?
polySUMO-protein conjugate + H2O
SUMO + SUMO-SUMO + SUMO-SUMO-SUMO + truncated polySUMO-protein conjugate
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?
polySUMO-protein conjugate + H2O
SUMO + SUMO-SUMO + SUMO-SUMO-SUMO + truncated polySUMO-protein conjugate
Ulp2 is capable of cleaving SUMO chains
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?
polySUMO-protein conjugate + H2O
SUMO + truncated polySUMO-protein conjugate + H2O
Ulp2 can cleave the SUMO-SUMO linkage in poly-SUMO chains
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?
polySUMO-protein conjugate + H2O
SUMO + truncated polySUMO-protein conjugate + H2O
Ulp2 can cleave the SUMO-SUMO linkage in poly-SUMO chains. The C-terminal noncatalytic domain of Ulp2 is required for efficient depolymerization of large poly-SUMO conjugates in vivo
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?
Smt3-modified protein conjugate + H2O
Smt3 + protein
Ulp2 is able to cleave isopeptide-linked Smt3 molecules from natural substrates. Smt3-protein deconjugation by Ulp2 is important for normal meiotic development
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?
Smt3-modified protein conjugate + H2O
Smt3 + protein
Ulp2 is able to cleave isopeptide-linked Smt3 molecules from natural substrates
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?
additional information
?
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Ulp2 is required for cell division following termination of the DNA damage checkpoint in Sacharomyces cerevisiae. The DNA damage checkpoint is a crucial defense mechanism used by cells to withstand DNA damage. Activation of the checkpoint halts the cell cycle at metaphase and allows time for DNA repair prior to cell division
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?
additional information
?
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Ulp2 SUMO protease is required for proper spindle dynamics during cell cycle resumption following a DNA damage-induced cell cycle arrest. It is proposed that one or more proteins is sumoylated following DNA damage-induced checkpoint activation, and this substrate(s) must be desumoylated by Ulp2 for proper cell cycle resumption
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?
additional information
?
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Ulp2 SUMO protease is required for proper spindle dynamics during cell cycle resumption following a DNA damage-induced cell cycle arrest. It is proposed that one or more proteins is sumoylated following DNA damage-induced checkpoint activation, and this substrate(s) must be desumoylated by Ulp2 for proper cell cycle resumption
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?
additional information
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isozyme Ulp2 is important for the control of poly-SUMO conjugates in cells and to dismantle SUMO chains in vitro. Isozyme Ulp2 acts sequentially rather than stochastically, processing substrate-linked poly-SUMO chains from their distal ends down to two linked SUMO moieties. Three linked SUMO units are the minimum length of a substrate-linked chain required for efficient binding to and processing by isozyme Ulp2, that disassembles SUMO chains by removing one SUMO moiety at a time from their ends, i.e. exo mechanism. Isozyme Ulp2 recognizes surfaces at or near the N-terminus of the distal SUMO moiety, as attachments to this end significantly reduce cleavage efficiency. Recombinant construction and expression of generate SMT3-led constructs, overview. Isozyme Ulp2 has three Smt3 binding sites that need to be occupied simultaneously to achieve full cooperative binding. Substrates equipped with mono- or di-Smt3, in contrast, are hardly bound if at all, substrate specificity analysis of immobilized recombinant enzyme, overview
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