3.4.22.B63: sortase C

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For detailed information about sortase C, go to the full flat file.

Reaction

The enzyme from Bacillus anthracis cleaves the LPNT-/-A sorting signal of BasH and BasI, thereby anchoring both polypeptides to the cell wall envelope of sporulating bacilli [17074072]. The enzymes from Streptococcus pneumoniae and Enterococcus faecalis are required for efficient pilus assembly. =

Synonyms

pilus-associated sortase C, sortase C, sortase C1, sortase C2, sortaseC1, Srt-2, SrtC, SrtC-1, SrtC-2, SrtC-3, SrtC1, SrtC2, SrtC_1, SrtC_2, yhhA

ECTree

     3 Hydrolases

         3.4 Acting on peptide bonds (peptidases)

             3.4.22 Cysteine endopeptidases

                3.4.22.B63 sortase C

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Results	in table
5	AA Sequence
8	Cloned(Commentary)
9	Crystallization (Commentary)
4	General Information
2	Inhibitors
12	kcat/KM [mM/s]
14	KM Value [mM]
2	Localization
1	Metals/Ions
2	Molecular Weight [Da]
16	Natural Substrates/ Products (Substrates)
28	Organism
8	Protein Variants
7	Purification (Commentary)
1	Reaction
23	Reference
1	Source Tissue
20	Substrates and Products (Substrate)
5	Subunits
40	Synonyms
12	Turnover Number [1/s]

Cloned

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Cloned on EC 3.4.22.B63 - sortase C

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CLONED (Commentary)

ORGANISM

UNIPROT

LITERATURE

expressed in Escherichia coli

Lactococcus lactis

Q9CHH2

732715

expressed in Escherichia coli B834(DE3) cells

Streptococcus agalactiae serogroup V

Q8E0S7

718001

expressed in Escherichia coli BL21(DE3) cells

Streptococcus suis

A4VY75

718380

expressed in Escherichia coli BL21(DE3) pLysS

Streptococcus pneumoniae

A0A0H2UNU0

709531

expressed in Escherichia coli M15 [pREP4] cells

Streptococcus agalactiae

-

718286

expressed in Escherichia coli Rosetta (DE3) pLysS cells

Streptococcus agalactiae serogroup V

Q8E0S7

732709

SrtC protein is cloned into plasmid pET24c and expressed in Escherichia coli without any tag

Streptococcus pneumoniae

-

690285

SrtCDELTAN (with a replacement of the N-terminal signal peptide of sortase C for six histidy) is expressed in Escherichia coli

Bacillus anthracis

-

694214