3.4.22.B6: cathepsin B-like protease
This is an abbreviated version!
For detailed information about cathepsin B-like protease, go to the full flat file.
Reaction
the enzyme does not readily hyrolyze benzyloxycarbonyl-Arg-Arg-7-amido-4-methylcoumarin (which is cleaved by cathespsin B). The enzyme is not able to cleave synthetic substrates having an Arg in position P2
=
Synonyms
AC-5 cathepsin B-like protease, CatB, CatB2, CathB-1, CathB2, CathB3, cathepsin B type protease, cathepsin B-like cysteine protease, cathepsin B-like cysteine protease 1, cathepsin B-like cysteine protease 2, cathepsin B-like cysteine proteinase, cathepsin B-like cysteine proteinase 1, cathepsin B-like cysteine proteinase 2, cathepsin B-like peptidase, cathepsin B-like protease, CBc1, CBc6, CBL1, CmCatB, CmCatB1, CmCatB2, CPB, CPC, CPC protease, eimeripain, HCB, LmacatB, LOC103512321, More, TbCatB, TcoCBc1, TcoCBc6, TrCB2
ECTree
Molecular Weight
Molecular Weight on EC 3.4.22.B6 - cathepsin B-like protease
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
24000
-
x * 24000, 2-D electrophoresis
27400
-
x * 37800, about, preproprotein, sequence calculation, x * 27400, about, mature protein, sequence calculation
28500
x * 28500, calculated for mature enzyme, x * 32000, SDS-PAGE (glycosylated)
32000
x * 28500, calculated for mature enzyme, x * 32000, SDS-PAGE (glycosylated)
35000
x * 35000, x * 45000, gelatin SDS-PAGE digestion assay
37000
-
x * 37000, recombinant detagged enzyme, SDS-PAGE
37800
-
x * 37800, about, preproprotein, sequence calculation, x * 27400, about, mature protein, sequence calculation
42000
x * 42000, recombinant zymogen, SDS-PAGE
43000
x * 43000, SDS-PAGE, predicted: 36700 Da (with pro-region and without signal peptide), 29000 Da (without pro-region)
45000
x * 35000, x * 45000, gelatin SDS-PAGE digestion assay
additional information
-
SDS-PAGE analysis of the eluted recombinant protein reveals a prominent band of 39000 Da and a minor protein of 40000 Da. These bands correspond to glycosylated forms of a 35000 Da protein, the expected size of the protease
additional information
SDS-PAGE analysis of the eluted recombinant protein reveals a prominent band of 39000 Da and a minor protein of 40000 Da. These bands correspond to glycosylated forms of a 35000 Da protein, the expected size of the protease
additional information
-
SDS-PAGE analysis of the eluted recombinant protein reveals a prominent band of 40000 Da and a minor protein of 50000 Da. These bands correspond to glycosylated forms of a 36000 Da protein, the expected size of the protease
additional information
SDS-PAGE analysis of the eluted recombinant protein reveals a prominent band of 40000 Da and a minor protein of 50000 Da. These bands correspond to glycosylated forms of a 36000 Da protein, the expected size of the protease