3.4.22.B49: cathepsin L1
This is an abbreviated version!
For detailed information about cathepsin L1, go to the full flat file.
Word Map on EC 3.4.22.B49
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3.4.22.B49
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cathepsins
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fasciola
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hepatica
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fluke
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fasciolosis
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metacercariae
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helminth
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gigantica
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medicine
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excysted
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trematode
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excretory-secretory
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immunodiagnosis
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agriculture
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mimotopes
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diagnostics
- 3.4.22.B49
- cathepsins
- fasciola
- hepatica
- fluke
-
fasciolosis
-
metacercariae
-
helminth
- gigantica
- medicine
-
excysted
-
trematode
-
excretory-secretory
-
immunodiagnosis
- agriculture
-
mimotopes
- diagnostics
Reaction
clear preference for Arg at P1 position (Lys, Glu, Thr, and Met are less efficient). FheCL1 shows distinct preference for hydrophobic amino acids in the P2, Leu is favored. Cathepsin L1 can accommodate Pro in the P2 position, but less efficiently than cathepsin L2. FheCL1 produces clear degradation fragments from collagen =
Synonyms
cat-L1H, cathepsin L1, cathepsin L1 cysteine protease, cathepsin L1 protease, cathepsin L1 proteinase, cathepsin L1g, cathepsin L1H, CatL1, CgCTSL1, CGI_10027418, CL1, CPFhW, CTSL1, cysteine proteinase 3, Da-CTSL1, FgCatL1H, FhCL1, FheCL1, FhpCL1
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Purification
Purification on EC 3.4.22.B49 - cathepsin L1
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gel filtration, proenzyme purified by affinity Ni-NTA-agarose chromatography
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in the presence of 1% Triton X-100 by Ni-NTA affinity column chromatography and Sephacryl S-100 gel filtration
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recombinant GST- and His6-tagged proCTSL1 enzyme from Escherichia coli strain Rosetta (DE3) by nickel affinity chromatography and gel filtration
recombinant His-tagged chimeric mutant from Escherichia coli strain Rosetta (DE3) by nickel affinity chromatography and dialysis
recombinant His-tagged enzyme from Escherichia coli strain Rosetta (DE3) by nickel affinity chromatography and two steps of dialysis
recombinant solubilized and refolded His-tagged enzyme from Escherichia coli by nickel affinity chromatography