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3.4.22.7: asclepain

This is an abbreviated version!
For detailed information about asclepain, go to the full flat file.

Word Map on EC 3.4.22.7

Reaction

Similar to that of papain =

Synonyms

asclepain c, asclepain cI, asclepain cII, asclepain f, papain-like endopeptidase, papain-like protease

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.22 Cysteine endopeptidases
                3.4.22.7 asclepain

Substrates Products

Substrates Products on EC 3.4.22.7 - asclepain

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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
azocasein + H2O
?
show the reaction diagram
-
-
-
-
?
benzoyl-L-Arg-p-nitroanilide + H2O
benzoyl-L-Arg + 4-nitroaniline
show the reaction diagram
-
-
-
-
?
casein + H2O
?
show the reaction diagram
L-pyroglutamyl-L-phenylalanyl-L-leucine 4-nitroanilide + H2O
L-pyroglutamyl-L-phenylalanyl-L-leucine + 4-nitroaniline
show the reaction diagram
very low activity
-
-
?
L-pyroglutamyl-L-phenylalanyl-L-leucine-4-nitroanilide + H2O
?
show the reaction diagram
-
-
-
-
?
L-pyroglutamyl-Phe-Leu-p-nitroanilide + H2O
L-pyroglutamyl-Phe-Leu + p-nitroaniline
show the reaction diagram
-
-
-
-
?
N-acetyl-L-tryptophanamide + H2O
N-acetyl-L-Trp + NH3
show the reaction diagram
N-alpha-benzyloxycarbonyl-Gly-4-nitrophenyl ester + H2O
N-alpha-benzyloxycarbonyl-Gly + 4-nitrophenol
show the reaction diagram
-
-
-
?
N-alpha-benzyloxycarbonyl-L-Ala-4-nitrophenyl ester + H2O
N-alpha-benzyloxycarbonyl-L-Ala + 4-nitrophenol
show the reaction diagram
-
-
-
?
N-alpha-benzyloxycarbonyl-L-Asn-4-nitrophenyl ester + H2O
N-alpha-benzyloxycarbonyl-L-Asn + 4-nitrophenol
show the reaction diagram
-
-
-
?
N-alpha-benzyloxycarbonyl-L-Asp-4-nitrophenyl ester + H2O
N-alpha-benzyloxycarbonyl-L-Asp + 4-nitrophenol
show the reaction diagram
asclepain cII has highest endoesterolytic activity using N-alpha-benzyloxycarbonyl-amino acid p-nitrophenyl esters with L-Asp as amino acid
-
-
?
N-alpha-benzyloxycarbonyl-L-Gln-4-nitrophenyl ester + H2O
N-alpha-benzyloxycarbonyl-L-Gln + 4-nitrophenol
show the reaction diagram
-
-
-
?
N-alpha-benzyloxycarbonyl-L-Tyr-4-nitrophenyl ester + H2O
N-alpha-benzyloxycarbonyl-L-Tyr + 4-nitrophenol
show the reaction diagram
-
-
-
?
N-Benzoyl-L-Arg ethyl ester + H2O
N-Benzoyl-L-Arg + ethanol
show the reaction diagram
N-carbobenzoxy-Gly p-nitrophenyl ester + H2O
N-carbobenzoxy-Gly + p-nitrophenol
show the reaction diagram
-
-
-
-
?
N-carbobenzoxy-L-Ala 4-nitrophenyl ester + H2O
N-carbobenzoxy-L-Ala + 4-nitrophenol
show the reaction diagram
-
47% of the activity with N-carbobenzoxy-L-Gln 4-nitrophenyl ester
-
-
?
N-carbobenzoxy-L-Gln 4-nitrophenyl ester + H2O
N-carbobenzoxy-L-Gln + 4-nitrophenol
show the reaction diagram
-
-
-
-
?
N-carbobenzoxy-L-Ile 4-nitrophenyl ester + H2O
N-carbobenzoxy-L-Ile + 4-nitrophenol
show the reaction diagram
-
59% of the activity with N-carbobenzoxy-L-Gln 4-nitrophenyl ester
-
-
?
N-carbobenzoxy-L-Lys 4-nitrophenyl ester + H2O
N-carbobenzoxy-L-Lys + 4-nitrophenol
show the reaction diagram
-
78% of the activity with N-carbobenzoxy-L-Gln 4-nitrophenyl ester
-
-
?
N-carbobenzoxy-L-Tyr 4-nitrophenyl ester + H2O
N-carbobenzoxy-L-Tyr + 4-nitrophenol
show the reaction diagram
-
51% of the activity with N-carbobenzoxy-L-Gln 4-nitrophenyl ester
-
-
?
N-carbobenzoxy-Leu p-nitrophenyl ester + H2O
N-carbobenzoxy-Leu + p-nitrophenol
show the reaction diagram
-
-
-
-
?
N-carbobenzoxy-Lys p-nitrophenyl ester + H2O
N-carbobenzoxy-Lys + 4-nitrophenol
show the reaction diagram
-
-
-
-
?
N-carbobenzoxy-Tyr p-nitrophenyl ester + H2O
N-carbobenzoxy-Tyr + p-nitrophenol
show the reaction diagram
-
-
-
-
?
N-methanesulfonylglycine 3-bromophenyl ester + H2O
N-methanesulfonylglycine + 3-bromophenol
show the reaction diagram
Asclepias sp.
-
-
-
-
?
N-methanesulfonylglycine 3-chlorophenyl ester + H2O
N-methanesulfonylglycine + 3-chlorophenol
show the reaction diagram
Asclepias sp.
-
-
-
-
?
N-methanesulfonylglycine 3-fluorophenyl ester + H2O
N-methanesulfonylglycine + 3-fluorophenol
show the reaction diagram
Asclepias sp.
-
-
-
-
?
N-methanesulfonylglycine 3-methylphenyl ester + H2O
N-methanesulfonylglycine + 3-methylphenol
show the reaction diagram
Asclepias sp.
-
-
-
-
?
N-methanesulfonylglycine 3-nitrophenyl ester + H2O
N-methanesulfonylglycine 3-nitrophenol
show the reaction diagram
Asclepias sp.
-
-
-
-
?
N-methanesulfonylglycine 3-tert-butylphenyl ester + H2O
N-methanesulfonylglycine + 3-tert-butylphenol
show the reaction diagram
Asclepias sp.
-
-
-
-
?
N-methanesulfonylglycine 4-bromophenyl ester + H2O
N-methanesulfonylglycine + 4-bromophenol
show the reaction diagram
Asclepias sp.
-
-
-
-
?
N-methanesulfonylglycine 4-chlorophenyl ester + H2O
N-methanesulfonylglycine + 4-chlorophenol
show the reaction diagram
Asclepias sp.
-
-
-
-
?
N-methanesulfonylglycine 4-fluorophenyl ester + H2O
N-methanesulfonylglycine + 4-fluorophenol
show the reaction diagram
Asclepias sp.
-
-
-
-
?
N-methanesulfonylglycine 4-methylphenyl ester + H2O
N-methanesulfonylglycine + 4-methylphenol
show the reaction diagram
Asclepias sp.
-
-
-
-
?
N-methanesulfonylglycine 4-nitrophenyl ester + H2O
N-methanesulfonylglycine + 4-nitrophenol
show the reaction diagram
Asclepias sp.
-
-
-
-
?
Nalpha-carbobenzoxy-Gly-4-nitrophenyl ester + H2O
Nalpha-carbobenzoxy-Gly + 4-nitrophenol
show the reaction diagram
-
about 25% activity compared to Nalpha-carbobenzoxy-L-Ala-4-nitrophenyl ester
-
-
?
Nalpha-carbobenzoxy-L-Ala-4-nitrophenyl ester + H2O
Nalpha-carbobenzoxy-L-Ala + 4-nitrophenol
show the reaction diagram
-
100% activity
-
-
?
Nalpha-carbobenzoxy-L-Gln-4-nitrophenyl ester + H2O
Nalpha-carbobenzoxy-L-Gln + 4-nitrophenol
show the reaction diagram
-
about 85% activity compared to Nalpha-carbobenzoxy-L-Ala-4-nitrophenyl ester
-
-
?
Nalpha-carbobenzoxy-L-Tyr-4-nitrophenyl ester + H2O
Nalpha-carbobenzoxy-L-Tyr + 4-nitrophenol
show the reaction diagram
-
about 20% activity compared to Nalpha-carbobenzoxy-L-Ala-4-nitrophenyl ester
-
-
?
Nalpha-CBZ-L-Gln-4-nitrophenyl ester + H2O
Nalpha-CBZ-L-Gln + 4-nitrophenol
show the reaction diagram
when assayed on N-alpha-CBZ-amino acid-4-nitrophenyl esters, the enzyme exhibits higher preference for the glutamine derivative
-
-
?
oxidized insulin B chain + H2O
?
show the reaction diagram
pGlu-Phe-Leu 4-nitroanilide + H2O
pGlu-Phe-Leu + 4-nitroaniline
show the reaction diagram
-
-
-
?
pGlu-Phe-Leu-p-nitroanilide + H2O
pGlu-Phe-Leu + p-nitroaniline
show the reaction diagram
-
-
-
?
Phe-Val-Asn-Ala-His-Leu-CysA-Gly-Ser-His-Leu-Val-Glu-Ala-Leu-Tyr-Leu-Val-CysA-Gly-Glu-Arg-Gly-Phe-Phe-Tyr-Thr-Pro-Lys-Ala + H2O
Phe-Val-Asn + Ser-His-Leu-Val-Glu-Ala-Leu + Leu-Val-Glu-Ala-Leu-Tyr-Leu-Val-CysA-Gly-Glu + Glu-Arg-Gly-Phe-Phe-Tyr-Thr-Pro-Lys-Ala + Gly-Phe-Phe-Tyr-Thr-Pro-Lys-Ala + Gly-Phe-Phe-Tyr-Thr
show the reaction diagram
-
-
14% Phe-Val-Asn + 22% Ser-His-Leu-Val-Glu-Ala-Leu + 15% Leu-Val-Glu-Ala-Leu-Tyr-Leu-Val-CysA-Gly-Glu + 10% Glu-Arg-Gly-Phe-Phe-Tyr-Thr-Pro-Lys-Ala + 10% Gly-Phe-Phe-Tyr-Thr-Pro-Lys-Ala + 13% Gly-Phe-Phe-Tyr-Thr
?
soybean protein + H2O
?
show the reaction diagram
the enzyme is able to selectively hydrolyze soybean proteins at pH 10, employing an enzyme/substrate ratio of 0.2% (w/w). The enzymatic hydrolysis allows a strong increase in the solubility, water and oil holding capacity
-
-
?
Z-Arg-p-nitroanilide + H2O
Z-Arg + p-nitroaniline
show the reaction diagram
-
-
-
-
?
additional information
?
-