3.4.22.7: asclepain

This is an abbreviated version!
For detailed information about asclepain, go to the full flat file.

Similar to that of papain =

asclepain c, asclepain cI, asclepain cII, asclepain f, papain-like endopeptidase, papain-like protease

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Results	in table
3	AA Sequence
2	Application
1	CAS Registry Number
1	Cloned(Commentary)
1	Crystallization (Commentary)
1	General Information
9	Inhibitors
1	kcat/KM [mM/s]
15	KM Value [mM]
2	Localization
21	Molecular Weight [Da]
4	Natural Substrates/ Products (Substrates)
20	Organism
7	pH Optimum
3	pH Range
6	pH Stability
6	pI Value
1	Posttranslational Modification
8	Purification (Commentary)
1	Reaction
1	Reaction Type
18	Reference
19	Source Tissue
6	Specific Activity [micromol/min/mg]
59	Substrates and Products (Substrate)
16	Subunits
14	Synonyms
1	Temperature Optimum [°C]
8	Temperature Stability [°C]
12	Turnover Number [1/s]

KM Value on EC 3.4.22.7 - asclepain

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0.73 - 0.82

L-pyroglutamyl-L-phenylalanyl-L-leucine-4-nitroanilide

0.1634

N-alpha-benzyloxycarbonyl-L-Gln-4-nitrophenyl ester

at 37°C, in 0.1 M Tris-HCl buffer (pH 8.0) containing 2 mM EDTA and 25 mM cysteine

0.0097 - 0.0389

N-carbobenzoxy-Lys p-nitrophenyl ester

0.163

Nalpha-CBZ-L-Gln-4-nitrophenyl ester

pH 8.5, 42°C

additional information

Asclepias sp.

Km-values for hydrolysis of aryl mesyl glycinates