3.4.22.7: asclepain

This is an abbreviated version!
For detailed information about asclepain, go to the full flat file.

Reaction

Similar to that of papain =

Synonyms

asclepain c, asclepain cI, asclepain cII, asclepain f, papain-like endopeptidase, papain-like protease

ECTree

     3 Hydrolases

         3.4 Acting on peptide bonds (peptidases)

             3.4.22 Cysteine endopeptidases

                3.4.22.7 asclepain

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Results	in table
3	AA Sequence
2	Application
1	CAS Registry Number
1	Cloned(Commentary)
1	Crystallization (Commentary)
1	General Information
9	Inhibitors
1	kcat/KM [mM/s]
15	KM Value [mM]
2	Localization
21	Molecular Weight [Da]
4	Natural Substrates/ Products (Substrates)
20	Organism
7	pH Optimum
3	pH Range
6	pH Stability
6	pI Value
1	Posttranslational Modification
8	Purification (Commentary)
1	Reaction
1	Reaction Type
18	Reference
19	Source Tissue
6	Specific Activity [micromol/min/mg]
59	Substrates and Products (Substrate)
16	Subunits
14	Synonyms
1	Temperature Optimum [°C]
8	Temperature Stability [°C]
12	Turnover Number [1/s]

Inhibitors

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of the Enzyme Summary Page.

Inhibitors on EC 3.4.22.7 - asclepain

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INHIBITOR

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

IMAGE

(2S,3S)-3-(N-[(S)-1-[N-(4-guanidinobutyl)carbamoyl]3-methylbutyl]carbamoyl)oxirane-2-carboxylic acid

Asclepias curassavica

B7VF64, B7VF65

i.e. E-64, complete immediate and irreversible inhibition at 10 mM after 30 min incubation

695296

E-64

2 entries

iodoacetic acid

Asclepias syriaca

-

-

81574

NaCl

Asclepias curassavica

-

1 M, 70% loss of activity

670802

PCMB

Asclepias syriaca

-

-

81574

phenylmethylsulfonyl fluoride

Asclepias curassavica

B7VF64, B7VF65

incubation with 100 mM phenylmethylsulfonyl fluoride for 30 min decreases activity to 63% of the initial value, but inhibition is partially reverted when 12 mM cysteine is added to the reaction mixture

695296

Sodium tetrathionate

Asclepias syriaca

-

-

81574

additional information

3 entries

-

E-64

Asclepias curassavica

-

-

670802, 695296

E-64

Asclepias curassavica

B7VF64, B7VF65

-

695296

additional information

Asclepias curassavica

-

1,10-phenantroline and pepstatine do not affect the activity of asclepain cII; activity of asclepain cII is inhibited by cysteine proteases inhibitors like E-64, but not by any other protease inhibitors such as 1,10-phenantroline, phenylmethanesulfonyl fluoride, and pepstatine

695296

-

additional information

Asclepias curassavica

B7VF64

1,10-phenantroline and pepstatine do not affect the activity of asclepain cII; activity of asclepain cII is inhibited by cysteine proteases inhibitors like E-64, but not by any other protease inhibitors such as 1,10-phenantroline, phenylmethanesulfonyl fluoride, and pepstatine

695296

-

additional information

Asclepias curassavica

B7VF65

1,10-phenantroline and pepstatine do not affect the activity of asclepain cII; activity of asclepain cII is inhibited by cysteine proteases inhibitors like E-64, but not by any other protease inhibitors such as 1,10-phenantroline, phenylmethanesulfonyl fluoride, and pepstatine

695296

-