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3.4.22.69: SARS coronavirus main proteinase

This is an abbreviated version!
For detailed information about SARS coronavirus main proteinase, go to the full flat file.

Word Map on EC 3.4.22.69

Reaction

TSAVLQSGFRK-NH2
+
H2O
=
TSAVLQ
 +
SGFRK-NH2

Synonyms

2019-nCoV 3C-like protease, 3C-like cysteine protease, 3C-like peptidase, 3C-like protease, 3CL protease, 3CL-pro, 3cLpro, C30.004, chymotrypsin-like protease, coronavirus 3C-like protease, coronavirus M(pro), coronavirus main protease, main protease, MERS-CoV 3C-like protease, Mpro, nsp5, porcine transmissible gastroenteritis virus Mpro, protease Mpro, SARS 3C-like protease, SARS 3C-like proteinase, SARS 3CL protease, SARS 3CLpro, SARS coronavirus 3C-like protease, SARS coronavirus 3C-like proteinase, SARS coronavirus 3CL protease, SARS coronavirus main peptidase, SARS coronavirus main protease, SARS coronavirus main proteinase, SARS coronavirus Mpro, SARS CoV main proteinase, SARS CoVMpro, SARS main protease, SARS Mpro, SARS-3CL protease, SARS-3CLpro, SARS-coronavirus 3CL protease, SARS-coronavirus main protease, SARS-CoV 3C-like peptidase, SARS-CoV 3C-like protease, SARS-CoV 3CL peptidase, SARS-CoV 3CL protease, SARS-CoV 3CLpro, SARS-CoV 3CLpro enzyme, SARS-CoV 3CLpro protease, SARS-CoV main protease, SARS-CoV Mpro, SARS-CoV nsp5-pp1a/pp1ab 3CL protease, SARS-CoV-2 3C-like protease, SARS-CoV-2 main protease, SARSCoV main protease, SARSCoV Mpro, severe acute respiratory syndrome coronavirus 3C-like protease, severe acute respiratory syndrome coronavirus 3CLpro, severe acute respiratory syndrome coronavirus main protease, severe acute respiratory syndrome coronavirus main proteinase, severe acute respiratory syndrome coronavirus Mpro, TGEV Mpro

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.22 Cysteine endopeptidases
                3.4.22.69 SARS coronavirus main proteinase

KM Value

KM Value on EC 3.4.22.69 - SARS coronavirus main proteinase

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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.306
(Ala-Arg-Leu-Gln-NH)2-rhodamine
-
rate of hydrolysis measured by change in absorbance at 496 nm
0.0017 - 0.023
Abz-LQSGFRK(Dnp)NH2
0.0005
Abz-LYQPPQTSITSAVLQSGFRK(Dnp)NH2
-
in the presence of 1 M sodium sulfate, pH 7.5 in 50 mM Tris-HCl buffer with 1 mM dithiothreitol, at 25°C
0.011
Abz-QTSITSAVLQSGFRK(Dnp)NH2
-
in the presence of 1 M sodium sulfate, pH 7.5 in 50 mM Tris-HCl buffer with 1 mM dithiothreitol, at 25°C
0.0024 - 0.016
Abz-SAVLQSGFRK(Dnp)NH2
0.0013
Abz-SAVLQSGFRKMAFPSGK(Dnp)NH2
-
in the presence of 1 M sodium sulfate, pH 7.5 in 50 mM Tris-HCl buffer with 1 mM dithiothreitol, at 25°C
0.002
Abz-SAVLQSGFRKMAK(Dnp)NH2
-
in the presence of 1 M sodium sulfate, pH 7.5 in 50 mM Tris-HCl buffer with 1 mM dithiothreitol, at 25°C
0.0016 - 0.0136
Abz-SAVLQSGK(Dnp)NH2
0.0011
Abz-SGADVLYQPPQTSITSAVLQSGFRK(Dnp)NH2
-
in the presence of 1 M sodium sulfate, pH 7.5 in 50 mM Tris-HCl buffer with 1 mM dithiothreitol, at 25°C
0.0013 - 0.033
Abz-VLQSGFRK(Dnp)NH2
1.44
ATVRLQAGNAT
-
pH 7.3, room temperature
0.05642 - 0.216
Dabcyl-KTSAVLQSGFRKME-EDANS
0.0245
Dabcyl-KTSAVLQSGFRKME-EDANS-amide
in 40 mM Tris-HCl buffer, pH 7.3, temperature not specified in the publication
0.0423 - 0.0787
DABCYL-TSAVLQSGFRKME-EDANS
-
0.549
FYPKLQASQAW
-
pH 7.3, room temperature
0.046
GPFVDRQTAQAAGTDT-NH2
-
pH 7.5, 37°C, mutant enzyme R188I
0.051
KVATVQSKMSD-NH2
-
pH 7.5, 37°C, mutant enzyme R188I
0.039
N-methylanthranilyl-TSAVLQSGFRK(2,4-dinitrophenyl)
pH 7.5, 30°C
0.004 - 0.037
o-aminobenzoyl-TSAVLQSGFRK-2,4-dinitrophenyl amide
0.146
o-aminobenzoyl-TSAVLQSGFRY(NO2)G
-
-
1.94
PHTVLQAVGAC
-
pH 7.3, room temperature
0.0186 - 0.0766
SAVLQMGFRK
0.0186 - 0.0766
Ser-Ala-Val-Leu-Gln-Met-Gly-Phe-Arg-Lys
0.583
SGVTFQGKFKK
-
pH 7.3, room temperature
0.073
SITSAVLQ-p-nitrophenyl ester
-
-
61.9
SWTSAVLQSGFRKWA
-
HPLC-based cleavage assay, measurement of fluorescence emission at 353 nm
0.286
TFTRLQSLENV
-
pH 7.3, room temperature
0.68 - 0.98
Thr-Ser-Ala-Val-Leu-Gln-4-nitroanilide
0.2226 - 0.3534
Thr-Ser-Ala-Val-Leu-Gln-p-nitroanilide
0.0338 - 1.15
TSAVLQSGFRK-NH2
0.03817 - 0.04938
[4-(4-dimethylaminophenylazo)benzoic acid]-KNSTLQSGLRKE-[5-[2'-(aminoethyl)amino]-naphthalenesulfonic acid]
0.045
[4-(4-dimethylaminophenylazo)benzoic acid]-KTSAVLQSGFRKME-5-['-(aminoethyl)amino]-naphthalenesulfonic acid
-
-
additional information
additional information
-
steady-state analysis of the solvent isotope effects on KM-value
-