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3.4.22.69: SARS coronavirus main proteinase

This is an abbreviated version!
For detailed information about SARS coronavirus main proteinase, go to the full flat file.

Word Map on EC 3.4.22.69

Reaction

TSAVLQSGFRK-NH2
+
H2O
=
TSAVLQ
 +
SGFRK-NH2

Synonyms

2019-nCoV 3C-like protease, 3C-like cysteine protease, 3C-like peptidase, 3C-like protease, 3CL protease, 3CL-pro, 3cLpro, C30.004, chymotrypsin-like protease, coronavirus 3C-like protease, coronavirus M(pro), coronavirus main protease, main protease, MERS-CoV 3C-like protease, Mpro, nsp5, porcine transmissible gastroenteritis virus Mpro, protease Mpro, SARS 3C-like protease, SARS 3C-like proteinase, SARS 3CL protease, SARS 3CLpro, SARS coronavirus 3C-like protease, SARS coronavirus 3C-like proteinase, SARS coronavirus 3CL protease, SARS coronavirus main peptidase, SARS coronavirus main protease, SARS coronavirus main proteinase, SARS coronavirus Mpro, SARS CoV main proteinase, SARS CoVMpro, SARS main protease, SARS Mpro, SARS-3CL protease, SARS-3CLpro, SARS-coronavirus 3CL protease, SARS-coronavirus main protease, SARS-CoV 3C-like peptidase, SARS-CoV 3C-like protease, SARS-CoV 3CL peptidase, SARS-CoV 3CL protease, SARS-CoV 3CLpro, SARS-CoV 3CLpro enzyme, SARS-CoV 3CLpro protease, SARS-CoV main protease, SARS-CoV Mpro, SARS-CoV nsp5-pp1a/pp1ab 3CL protease, SARS-CoV-2 3C-like protease, SARS-CoV-2 main protease, SARSCoV main protease, SARSCoV Mpro, severe acute respiratory syndrome coronavirus 3C-like protease, severe acute respiratory syndrome coronavirus 3CLpro, severe acute respiratory syndrome coronavirus main protease, severe acute respiratory syndrome coronavirus main proteinase, severe acute respiratory syndrome coronavirus Mpro, TGEV Mpro

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.22 Cysteine endopeptidases
                3.4.22.69 SARS coronavirus main proteinase

Cloned

Cloned on EC 3.4.22.69 - SARS coronavirus main proteinase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
E166A mutant protein expressed in Escherichia coli BL21(DE3)
-
expressed in Escherichia coli BL21 cells
-
expressed in Escherichia coli BL21 Star(DE3) cells
-
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli Rosetta (DE3) cells
-
expression in Escherichia coli
fused to maltose-binding protein and expressed in E. coli BL21
-
high level of expression of of proteolysis-resistant mutant R188I in Escherichia coli
-
His-tagged artificial polyprotein (cyan fluorescent protein-SARS-CoV 3CLpro-yellow fluorescent protein) expressed in Escherichia coli
-
His-tagged SARS-CoV 3CL protease expressed in Escherichia coli
-
mutant enzyme G11A and wild-type enzyme are expressed in Escherichia coli
-
R298A protease is expressed in Escherichia coli strain BL21(DE3)
-
recombinant expression of His-tagged mutant R298A enzyme in Escherichia coli strain BL21(DE3) from pET-28a(+) vector
the catalytic domain of 3CLpro is synthesised chemically and cloned into a bacteriophage T7-based expression vector, pBT7. The plasmid DNA is transformed into Escherichia coli BL21 (DE3) for protein expression
wild type and His-tagged T25G mutant are expressed in Escherichia coli cells
-
wild-type and mutant glutathione S-transferase-fusion constructs are transformed into Escherichia coli BL21 cell strain for overexpression
-
wild-type enzyme and C-terminally truncated proteases, expression in Escherichia coli
-