3.4.22.67: zingipain

This is an abbreviated version!
For detailed information about zingipain, go to the full flat file.

Reaction

preferential cleavage of peptides with a proline residue at the P2 position =

Synonyms

Bgp, C01.017, cysteine proteinase GP-II, F50, ginger protease, ginger protease II, GP-II, zingibain

ECTree

     3 Hydrolases

         3.4 Acting on peptide bonds (peptidases)

             3.4.22 Cysteine endopeptidases

                3.4.22.67 zingipain

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Results	in table
6	Activating Compound
8	AA Sequence
3	Application
1	CAS Registry Number
2	Crystallization (Commentary)
4	General Information
3	General Stability
38	Inhibitors
2	KM Value [mM]
10	Metals/Ions
6	Molecular Weight [Da]
14	Organism
5	pH Optimum
4	pH Range
1	pH Stability
1	pI Value
2	Posttranslational Modification
8	Purification (Commentary)
1	Reaction
1	Reaction Type
14	Reference
9	Source Tissue
3	Specific Activity [micromol/min/mg]
3	Storage Stability
21	Substrates and Products (Substrate)
4	Subunits
13	Synonyms
4	Temperature Optimum [°C]
2	Temperature Range [°C]
3	Temperature Stability [°C]

Purification

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Purification on EC 3.4.22.67 - zingipain

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PURIFICATION (Commentary)

ORGANISM

UNIPROT

LITERATURE

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Zingiber officinale

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647391

native enzyme 14.91fold from rhizomes by ammonium sulfate fractionation, tert-butanol precipitation at 25°C and pH 7.0 using the interfacial precipitateand the aqueous phase, and ultrafiltration, method optimization

Zingiber officinale

-

731939

native enzyme from rhizomes by ammonium sulfate fractionation and dialysis

Zingiber officinale

-

731925

native enzyme from rhizomes by ammonium sulfate fractionation, anion exchange chromatography, SDS-PAGE, and gel filtration

Zingiber ottensii

-

718310

native enzyme from rhizomes by saturation ammonium sulfate fractionation and anion exchange chromatography to homogeneity

Zingiber officinale

-

731160

partially purified 252fold with a recovery of 61%, ion exchange chromatography

Zingiber officinale

-

669676

purification via a three-phase partitioning system

Zingiber officinale

-

753364

ultrasonic-assisted liquid-liquid microextraction of the proteases from ginger and sodom apple using natural deep eutectic solvents, NADES. Selective partitioning of NADES-assisted microextraction yields more protease in NADES-enriched top phase. Maximum yield is achieved with 25% (v/v) of NADES, 15% (w/v) of source concentration and ultrasound temperature and time as 35°C and 10 min, respectively

Zingiber officinale

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754662