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3.4.22.59: caspase-6

This is an abbreviated version!
For detailed information about caspase-6, go to the full flat file.

Word Map on EC 3.4.22.59

Reaction

strict requirement for Asp at position P1 and has a preferred cleavage sequence of Val-Glu-His-Asp-/- =

Synonyms

apoptotic protease Mch-2, C14.005, Cas6, Casp-6, Casp.6, Casp6, caspase 6, caspase-6, caspase-6A, caspase-6B, Csp-6, Csp6, HLcaspase-6, MCH2, Pfcasp-6, VEIDase

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.22 Cysteine endopeptidases
                3.4.22.59 caspase-6

Purification

Purification on EC 3.4.22.59 - caspase-6

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
Hi-Trap nickel-affinity column chromatography
immobilized metal ion affinity chromatography (Ni2+), hydrophobic interaction chromatography, gel filtration
Ni-NTA agarose column chromatography
Ni-NTA agarose column chromatography, and gel filtration
purified to homogeneity
-
recombinant His-tagged apo-caspase-6 and caspase-6 from Escherichia coli by nickel affinity chromatography and gel filtration
recombinant His-tagged pro-Casp6b from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, recombinant catalytic mutant pro-Casp6aC163A from Escherichia coli strain BL21(DE3)
-