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3.4.22.59: caspase-6

This is an abbreviated version!
For detailed information about caspase-6, go to the full flat file.

Word Map on EC 3.4.22.59

Reaction

strict requirement for Asp at position P1 and has a preferred cleavage sequence of Val-Glu-His-Asp-/- =

Synonyms

apoptotic protease Mch-2, C14.005, Cas6, Casp-6, Casp.6, Casp6, caspase 6, caspase-6, caspase-6A, caspase-6B, Csp-6, Csp6, HLcaspase-6, MCH2, Pfcasp-6, VEIDase

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.22 Cysteine endopeptidases
                3.4.22.59 caspase-6

Cloned

Cloned on EC 3.4.22.59 - caspase-6

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
a caspase-6 with a 23-amino-acid deletion in the pro-domain is expressed in Escherichia coli BL21 Codon Plus (DE3) RIL cells
a His-tagged version (residues 24-293) expressed in Escherichia coli
Casp6 genetic organization, expression of His-tagged wild-type and mutant pro-caspase-6 in Escherichia coli and in HEK-293T cells
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caspase-6 expression analysis
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cloned into pET-23 bacterial expression vector
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expressed in Escherichia coli BL21(DE3) cells
expressed in Mus musculus
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expressed in Mus musculus hippocampus
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expression in Escherichia BL21(DE3)
expression in Escherichia coli
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expression in Escherichia coli BL21 (DE3)
expression in MDCK cells
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expression of His-tagged apo-caspase-6 and caspase-6 in Escherichia coli
inserted into the pIVEX vector
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recombinant DELTA23 caspase-6 and DELTA23 caspase-6 mutant C163S with C-terminal His-tag are expressed in Escherichia coli BL21 codon plus (DE3) RIL cells
the CASP6 gene generates an alternatively spliced transcript known as CASP6beta in addition to the CASP6alpha transcript that encodes pro-Casp6a. Individual expression of His-tagged pro-Casp6b and of catalytic mutant pro-Casp6aC163A in Escherichia coli strain BL21(DE3), Expression of pro-Casp6b from the pCep4beta construct in SK-N-SH cells
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wild-type enzyme and variants are expressed in Escherichia coli