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3.4.22.56: caspase-3

This is an abbreviated version!
For detailed information about caspase-3, go to the full flat file.

Word Map on EC 3.4.22.56

Reaction

strict requirement for an Asp residue at positions P1 and P4. It has a preferred cleavage sequence of Asp-Xaa-Xaa-Asp-/- with a hydrophobic amino-acid residue at P2 and a hydrophilic amino-acid residue at P3, although Val or Ala are also accepted at this position =

Synonyms

apopain, C14.003, Cas-3, CASP-3, CASP3, caspase 3, caspase-3, CgCaspase-3, CPP-32, CPP32, CPP32/apopain, cystein aspartic-specific protease-3, cysteine aspartic acid-specific protease, cysteine protease CPP32, DEVDase, IRP, Lyccasp3, Mncaspase-3c, More, SBTc-3, SCA-1, SREBP cleavage activity 1, Yama protein, Yama/CPP32, ZCASP3

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.22 Cysteine endopeptidases
                3.4.22.56 caspase-3

Cloned

Cloned on EC 3.4.22.56 - caspase-3

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
CASP3, expression analysis in melanoma cells
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caspase-3 expression analysis
caspase-3 expression analysis in CLL cells
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cloning of full-length cDNA of Lyccasp3, DNA and amino acid sequence determination and analysis, phylogenetic tree, real-time PCR expression analysis, functional recombinant expression in Escherichia coli strain BL21 (DE3)
engineering of a light-activated human caspase-3 (Caspase-LOV) by exploiting its natural spring-loaded activation mechanism through rational insertion of the light-sensitive LOV2 domain that expands upon illumination. Using the tissue-specific expression system (UAS)-GAL4, Caspase-LOV is specifically expressed in three neuronal cell types: retinal, sensory, and motor neuron of Drosophila
expressed in Escherichia coli
expressed in Escherichia coli Transetta (DE3) cells
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expression in Escherichia coli
expression in Escherichia coli strain BL21(DE3)
expression of FLAG-tagged caspase-3 in DKD cells. Simultaneous expression of HA-procaspase-3 and HA-procaspase-7, EC 3.4.22.60, in DKD HeLa cells together with FLAG-caspase-3 or FLAG-caspase-7. HA-procaspase-3 is coimmunoprecipitated with FLAG-procaspase-3 but not with FLAG-procaspase-7. Similarly, HA-procaspase-7 is co-immunoprecipitated with FLAG-procaspase-7 but not with FLAG-procaspase-3
expression of His6-tagged caspase-3 in Escherichia coli, effects of transiently-expressed VhhCasp31 and VhhCasp32 intrabodies on oxidative-stress-induced apoptosis in SHSY-5Y cells, overview
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from embryo
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GFP-tagged subunit and enzyme expression, the small subunit as well as the full-length caspase-3 display evident cytoplasm localization, while the large subunit and its truncated forms are distributed throughout the whole cell
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quantitative expression analysis in several ovarian and endometrial cancer cell lines by RT-PCR
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sequence encoding the caspase-3 catalytic domain (no prodomain) with a C-terminal hexa-His tag is inserted into vector pET-16b and expressed in Escherichia coli BL21
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the full-length caspase-3 gene containing an N-terminal 6His-tag is expressed in Escherichia coli