Any feedback?
Please rate this page
(all_enzymes.php)
(0/150)

BRENDA support

3.4.22.54: calpain-3

This is an abbreviated version!
For detailed information about calpain-3, go to the full flat file.

Word Map on EC 3.4.22.54

Reaction

broad endopeptidase activity =

Synonyms

C3DIV, calcium-activated neutral proteinase 3, Calp3, calpain 3, calpain 3 (p94), calpain 3 domain IV, calpain 3/p94, calpain L3, calpain M, calpain p94, calpain-3, calpain3, CANP 3, CAPN3, Cn94, MP78, Mp84, muscle calpain, muscle-specific calcium-activated neutral protease 3, muscle-specific calpain, nCL-1, p94, p94-calpain, p94/calpain 3, skeletal muscle-specific calpain

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.22 Cysteine endopeptidases
                3.4.22.54 calpain-3

Cloned

Cloned on EC 3.4.22.54 - calpain-3

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
cloning of human full-length CDSs in BD Creator(TM) system donor vector
COS-7 cells are transfected with C3 construct ssubcloned into pcDNA 3.1 and/or a C-terminal human FLNC construct subcloned into pCMV Tag2B
-
expressed in NIH-3T3 fibroblasts
expression in COS-7 cells
expression in Eschericia coli
-
expression of domain IV in Escherichia coli BL21 (DE3)
skeletal muscle enzyme
the C-terminally truncated form of the enzyme that comprises the protease cor, domains I and II, alone with its insertion sequence, IS1, and N-terminal leader sequence, NS is expressed in Escherichia coli BL21(DE3)
-
the His6-tagged PEF domain of calpain 3 coexpressed with the PEF domain of the small subunit that has been tagged with an antifreeze protein is expressed in Escherichia coli BL21(DE3) cells
-