3.4.22.49: separase
This is an abbreviated version!
For detailed information about separase, go to the full flat file.
Word Map on EC 3.4.22.49
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3.4.22.49
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chromatid
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sister
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anaphase
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cohesin
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mitosis
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securin
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spindle
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metaphase
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meiotic
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checkpoint
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centromeres
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kinetochore
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anaphase-promoting
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scc1
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prophase
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aneuploidy
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rec8
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cdc14
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rad21
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metaphase-to-anaphase
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centrosome
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shugoshins
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centriole
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sister-chromatid
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metaphase-anaphase
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disjunction
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medicaid
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disengagement
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prometaphase
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missegregation
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polo-like
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biorientation
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cyclosome
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kleisin
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meiosis-specific
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chiasmata
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pds1
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condensins
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medicine
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diagnostics
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nondisjunction
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molecular biology
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cdk1-dependent
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pp2acdc55
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kinetochore-microtubule
- 3.4.22.49
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chromatid
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sister
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anaphase
- cohesin
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mitosis
- securin
- spindle
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metaphase
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meiotic
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checkpoint
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centromeres
-
kinetochore
-
anaphase-promoting
- scc1
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prophase
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aneuploidy
- rec8
- cdc14
- rad21
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metaphase-to-anaphase
- centrosome
- shugoshins
- centriole
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sister-chromatid
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metaphase-anaphase
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disjunction
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medicaid
-
disengagement
-
prometaphase
-
missegregation
-
polo-like
-
biorientation
-
cyclosome
-
kleisin
-
meiosis-specific
-
chiasmata
- pds1
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condensins
- medicine
- diagnostics
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nondisjunction
- molecular biology
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cdk1-dependent
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pp2acdc55
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kinetochore-microtubule
Reaction
all bonds known to be hydrolysed by this endopeptidase have arginine in P1 and an acidic residue in P4. P6 is often occupied by an acidic residue or by an hydroxy-amino-acid residue, the phosphorylation of which enhances cleavage =
Synonyms
AESP, AtESP, Cut1, Cut1/separase, ESP, Esp1, ESPL1, sep-1, separase, separin, SSE, TbSep
ECTree
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Inhibitors
Inhibitors on EC 3.4.22.49 - separase
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1,3-dihydroxy-2,2-dimethyl-5-(trifluoromethyl)-2H-benzimidazole-1,3-diium
0.1 mM, 8.0% inhibition
1,3-dihydroxy-2,2-dimethyl-5-nitro-2H-benzimidazole-1,3-diium
0.1 mM, 95.9% inhibition
1,3-dihydroxy-2,2-dimethyl-5-[(trifluoromethoxy)sulfinyl]-2H-benzimidazole-1,3-diium
0.1 mM, 64.6% inhibition
1,3-dihydroxy-5-nitrospiro[benzimidazole-1,3-diium-2,1'-cyclohexane]
0.1 mM, 99.5% inhibition
1,3-dihydroxy-5-nitrospiro[benzimidazole-1,3-diium-2,1'-cyclopentane]
0.1 mM, 96.2% inhibition
1,3-dihydroxy-5-[hydroxy(methoxy)methyl]-2,2-dimethyl-2H-benzimidazole-1,3-diium
0.1 mM, 14.6% inhibition
2-(4-carboxyphenyl)-1,3-dihydroxy-2-methyl-5-nitro-2H-benzimidazole-1,3-diium
0.1 mM, 84.1% inhibition
2-butyl-1,3-dihydroxy-2-methyl-5-nitro-2H-benzimidazole-1,3-diium
0.1 mM, 99.8% inhibition
2-ethyl-1,3-dihydroxy-2-methyl-5-nitro-2H-benzimidazole-1,3-diium
0.1 mM, 99.6% inhibition
2-ethyl-1,3-dihydroxy-5-nitro-2-propyl-2H-benzimidazole-1,3-diium
0.1 mM, 99.2% inhibition
4-bromo-1,3-dihydroxy-2,2-dimethyl-6-nitro-2H-benzimidazole-1,3-diium
0.1 mM, 100% inhibition
5-(butylamino)-1,3-dihydroxy-2,2-dimethyl-2H-benzimidazole-1,3-diium
0.1 mM, 40.9% inhibition
5-bromo-1,3-dihydroxy-2,2-dimethyl-2H-benzimidazole-1,3-diium
0.1 mM, 24.6% inhibition
5-carboxy-1,3-dihydroxy-2,2-dimethyl-2H-benzimidazole-1,3-diium
0.1 mM, 24.4% inhibition
5-ethoxy-1,3-dihydroxy-2,2-dimethyl-2H-benzimidazole-1,3-diium
0.1 mM, 21.2% inhibition
5-ethoxy-6-fluoro-1,3-dihydroxy-2,2-dimethyl-2H-benzimidazole-1,3-diium
0.1 mM, 25.2% inhibition
5-fluoro-1,3-dihydroxy-2,2-dimethyl-2H-benzimidazole-1,3-diium
0.1 mM, 35.4% inhibition
5-fluoro-1,3-dihydroxy-2,2-dimethyl-6-nitro-2H-benzimidazole-1,3-diium
0.1 mM, 60.1% inhibition
5-hexyl-1,3-dihydroxy-2,2-dimethyl-2H-benzimidazole-1,3-diium
0.1 mM, 39.3% inhibition
Cdc55
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results suggest that Cdc55 acts as inhibitor downstream from shugoshin
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Cdk1 kinase
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phosphorylation-dependent binding of the cyclin B1 subunit of Cdk1 kinase to a Cdc6-like domain of separase inhibits separase
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cyclin-dependent kinase 1-cyclin B1
Cdk1-cyclin B1 triggers precipitation of separase by phosphorylation but stabilizes it by inhibitory binding. Only separase that is first complexed by Cdk1-cyclin B1 can later be activated by cyclin B1 degradation
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Pds1
Pds1 is a chaperone, inhibition of Esp1 by overexpression of undegradable Pds1 blocks mitotic exit via blockage of cohesin cleavage
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peptide
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acyloxymethyl ketone derivative of human SCC1 cleavage site peptide, chloromethyl ketone derivatives of the yeast Scc1 cleavage site
securin Pds1
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an inhibitor of separase Esp1 in budding yeast. As Pds1 is degraded, Esp1 is activated, and cells transit into anaphase
securing
the motif containing H134 in securing isoform PTTG1 has a strong affinity for separase and is involved in inhibiting it, while another domain(s) in isoform is involved in activating separase and has a weaker affinity for it. Isoform PTTG2 does not interact with separase
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sercurin
securin persistently binds and inhibits separase during much of metaphase
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shugoshin
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prevents separase activation independently of securin, protein phosphatase 2A coupled to regulatory subunit Cdc55 is essential for Shugoshin-mediated inhibition
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destruction of securin occurs only upon the correct attachment of chromosomes to the spindle
securin
in addition to its inhibitory role, can act as a molecular chaperone of separase, essential for its proper folding
securin
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in addition to its inhibitory role, can act as a molecular chaperone of separase, essential for its proper folding
securin
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in addition to its inhibitory role, can act as a molecular chaperone of separase, essential for its proper folding
securin
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in addition to its inhibitory role, can act as a molecular chaperone of separase, essential for its proper folding
securin
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securin homolog pimples, i.e. PIM, binds and inhibits separase
securin
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in addition to its inhibitory role, can act as a molecular chaperone of separase, essential for its proper folding
securin
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small protein that binds to and inhibits separase until all pairs of chromatids have established bipolar spindle attachments
securin
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securin inhibits separase by blocking the access of substrates to the active site
securin
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in addition to its inhibitory role, can act as a molecular chaperone of separase, essential for its proper folding. Securin is dispensable for the growth of normal human cells, in contrast to cancer cells, where depletion of PTTG1 leads to chromosome instability. The human separase-securin complex shows a whale-type distinct elongated pattern. In this complex, securin is thought to interact with the N-part of separase spanned by the ARM repeats. The N- to C-terminus intramolecular interaction in separase molecules is considered to be necessary for their catalytic activation, and this interaction is abolished by securin binding
securin
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in addition to its inhibitory role, can act as a molecular chaperone of separase, essential for its proper folding
securin
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in addition to its inhibitory role, can act as a molecular chaperone of separase, essential for its proper folding
securin
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small protein that binds to and inhibits separase until all pairs of chromatids have established bipolar spindle attachments
securin
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securin regulates both the proteolytic and non-proteolytic activities of separase
securin
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in addition to its inhibitory role, can act as a molecular chaperone of separase, essential for its proper folding. The first 156 amino acids of Esp1 seem imperative for the binding of securin Pds1, it interacts with other parts of Esp1 as well
securin
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small protein that binds to and inhibits separase until all pairs of chromatids have established bipolar spindle attachments
securin
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in addition to its inhibitory role, can act as a molecular chaperone of separase, essential for its proper folding. Interaction takes place between the N-terminus of separase and the C-terminus of securin
securin
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in addition to its inhibitory role, can act as a molecular chaperone of separase, essential for its proper folding
securin
Thermochaetoides thermophila
inhibits separase by binding as a pseudo substrate
securin
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in addition to its inhibitory role, can act as a molecular chaperone of separase, essential for its proper folding
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nuclear exclusion of separase might provide the means to preclude cohesin cleavage at telophase and G1 stage of the cell cycle
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additional information
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separase is kept inactive in human cells by Cdk(Cdc2)-dependent phosphorylation even when securin is degraded
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additional information
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nuclear exclusion of separase might provide the means to preclude cohesin cleavage at telophase and G1 stage of the cell cycle
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additional information
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cohesin cleavage is inhibited by a PP2ACdc55-dependent mechanism
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