3.4.22.49: separase
This is an abbreviated version!
For detailed information about separase, go to the full flat file.
Word Map on EC 3.4.22.49
-
3.4.22.49
-
chromatid
-
sister
-
anaphase
-
cohesin
-
mitosis
-
securin
-
spindle
-
metaphase
-
meiotic
-
checkpoint
-
centromeres
-
kinetochore
-
anaphase-promoting
-
scc1
-
prophase
-
aneuploidy
-
rec8
-
cdc14
-
rad21
-
metaphase-to-anaphase
-
centrosome
-
shugoshins
-
centriole
-
sister-chromatid
-
metaphase-anaphase
-
disjunction
-
medicaid
-
disengagement
-
prometaphase
-
missegregation
-
polo-like
-
biorientation
-
cyclosome
-
kleisin
-
meiosis-specific
-
chiasmata
-
pds1
-
condensins
-
medicine
-
diagnostics
-
nondisjunction
-
molecular biology
-
cdk1-dependent
-
pp2acdc55
-
kinetochore-microtubule
- 3.4.22.49
-
chromatid
-
sister
-
anaphase
- cohesin
-
mitosis
- securin
- spindle
-
metaphase
-
meiotic
-
checkpoint
-
centromeres
-
kinetochore
-
anaphase-promoting
- scc1
-
prophase
-
aneuploidy
- rec8
- cdc14
- rad21
-
metaphase-to-anaphase
- centrosome
- shugoshins
- centriole
-
sister-chromatid
-
metaphase-anaphase
-
disjunction
-
medicaid
-
disengagement
-
prometaphase
-
missegregation
-
polo-like
-
biorientation
-
cyclosome
-
kleisin
-
meiosis-specific
-
chiasmata
- pds1
-
condensins
- medicine
- diagnostics
-
nondisjunction
- molecular biology
-
cdk1-dependent
-
pp2acdc55
-
kinetochore-microtubule
Reaction
all bonds known to be hydrolysed by this endopeptidase have arginine in P1 and an acidic residue in P4. P6 is often occupied by an acidic residue or by an hydroxy-amino-acid residue, the phosphorylation of which enhances cleavage =
Synonyms
AESP, AtESP, Cut1, Cut1/separase, ESP, Esp1, ESPL1, sep-1, separase, separin, SSE, TbSep
ECTree
3.4.22.49 separaseAdvanced search results
results (
results (Activating Compound
Activating Compound on EC 3.4.22.49 - separase
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
top
ACTIVATING COMPOUND 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
IMAGE
cell division cycle 6
-
Cdc6, a mitotic substrate of polo-like kinase. Cleavage of chesin/Rad21 is much greater in wild-type Cdc6 expressing cells than in GFP and Cdc6-T37V mutant expressing cells
-
imatinib
activation of separase proteolytic activity occurs exclusively in BCR-ABL-positive cells during imatinib treatment (0.00025-0.01 mM for 24 h)
DNA
-
chromosomal DNA is required as a cofactor for the cleavage of cohesin to occur, and allows separase to selectively cleave only the chromosome-associated cohesin. Separase binds to DNA in a sequence nonspecific manner in vitro and associates with the entire length of the mitotic chromosomes
DNA
-
chromosomal DNA is required as a cofactor for the cleavage of cohesin to occur, and allows separase to selectively cleave only the chromosome-associated cohesin. Separase binds to DNA in a sequence nonspecific manner in vitro and associates with the entire length of the mitotic chromosomes
DNA
-
chromosomal DNA is required as a cofactor for the cleavage of cohesin to occur, and allows separase to selectively cleave only the chromosome-associated cohesin
securin
-
securin is required to support separase activity in anaphase
securin
-
the central domain of securin has a functionally essential specific sequence that may directly interact with the catalytic region of separase. This central securin domain is unrelated to destruction by polyubiquitination, but essential for the activation of separase
securing
the motif containing H134 in securing isoform PTTG1 has a strong affinity for separase and is involved in inhibiting it, while another domain(s) in isoform PTTG2 is involved in activating separase and has a weaker affinity for it. Isoform PTTG2 does not interact with separase
-
additional information
-
separase can be activated by incubating immunoglobulin G-Sepharose 6 containing ZZ TEV4-separase with anaphase-initiated Xenopus cytostatic factor to degrade its inhibitor securin at room temperature for 1 h
-
additional information
-
Cdc20 is essential for mitotic progression
-
additional information
-
most of the budding yeast cohesin is cleaved in anaphase, and this cleavage is stimulated by phosphorylation of the Scc1 subunit by the Plk1 kinase
-
