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3.4.22.48: staphopain

This is an abbreviated version!
For detailed information about staphopain, go to the full flat file.

Word Map on EC 3.4.22.48

Reaction

broad endopeptidase action on proteins including elastin, but rather limited hydrolysis of small-molecule substrates. Assays are conveniently made with hemoglobin, casein or Z-Phe-Arg-NHMec as substrate =

Synonyms

Ecp, extracellular cysteine protease, SCPA, Sscp A, Sscp B, SspB, SspB protein, staphopain, staphopain A, staphopain B, staphopain C, staphylopain

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.22 Cysteine endopeptidases
                3.4.22.48 staphopain

Purification

Purification on EC 3.4.22.48 - staphopain

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
cysteine protease StpA2aur CH-91 purified by gel filtration
-
from culture supernatant, apparant homogenity
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His-Select nickel affinity column chromatography
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ion-exchange chromatography, gelfiltration, immobilized metal ion affinity chromatography for His-tagged protein
native from culture supernatant
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purified by gel filtration
-
recombinant and native protein, gel filtration
recombinant co-expressed His-tagged staphopain A and inhibitor staphostatin A from Escherichia coli strain BL21(DE3) inclusion bodies by refolding and nickel affinity chromatography, method optimization
-
recombinant GST-fusion prostaphopain B from Escherichia coli strain BL21(DE3) by glutathione affinity chromatography, gel filtration, and cleavage of the GST-tag followed by ultrafiltration
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recombinant inactive mutant form, His-tagged, from Escherichia coli
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recombinant proenzyme form as GST-fusion protein from Escherichia coli, affinity chromatography, cleavage by V8 proteases to mature enzyme form
-
recombinant protein, purified by gel filtration
-