3.4.22.48: staphopain
This is an abbreviated version!
For detailed information about staphopain, go to the full flat file.
Word Map on EC 3.4.22.48
-
3.4.22.48
-
aureus
-
staphylococcal
-
streptococcal
-
pyogenes
-
staphostatins
-
speb
-
exotoxin
-
pyrogenic
-
aureolysin
-
sspc
-
gordonii
-
pharmacology
-
medicine
- 3.4.22.48
- aureus
-
staphylococcal
- streptococcal
- pyogenes
- staphostatins
- speb
-
exotoxin
-
pyrogenic
- aureolysin
- sspc
- gordonii
- pharmacology
- medicine
Reaction
broad endopeptidase action on proteins including elastin, but rather limited hydrolysis of small-molecule substrates. Assays are conveniently made with hemoglobin, casein or Z-Phe-Arg-NHMec as substrate =
Synonyms
Ecp, extracellular cysteine protease, SCPA, Sscp A, Sscp B, SspB, SspB protein, staphopain, staphopain A, staphopain B, staphopain C, staphylopain
ECTree
Advanced search results
Purification
Purification on EC 3.4.22.48 - staphopain
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
ion-exchange chromatography, gelfiltration, immobilized metal ion affinity chromatography for His-tagged protein
recombinant co-expressed His-tagged staphopain A and inhibitor staphostatin A from Escherichia coli strain BL21(DE3) inclusion bodies by refolding and nickel affinity chromatography, method optimization
-
recombinant GST-fusion prostaphopain B from Escherichia coli strain BL21(DE3) by glutathione affinity chromatography, gel filtration, and cleavage of the GST-tag followed by ultrafiltration
-
recombinant proenzyme form as GST-fusion protein from Escherichia coli, affinity chromatography, cleavage by V8 proteases to mature enzyme form
-