3.4.22.48: staphopain

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For detailed information about staphopain, go to the full flat file.

Reaction

broad endopeptidase action on proteins including elastin, but rather limited hydrolysis of small-molecule substrates. Assays are conveniently made with hemoglobin, casein or Z-Phe-Arg-NHMec as substrate =

Synonyms

Ecp, extracellular cysteine protease, SCPA, Sscp A, Sscp B, SspB, SspB protein, staphopain, staphopain A, staphopain B, staphopain C, staphylopain

ECTree

     3 Hydrolases

         3.4 Acting on peptide bonds (peptidases)

             3.4.22 Cysteine endopeptidases

                3.4.22.48 staphopain

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Results	in table
1	Activating Compound
63	AA Sequence
4	Application
1	CAS Registry Number
18	Cloned(Commentary)
4	Crystallization (Commentary)
2	Expression
8	General Information
36	Inhibitors
8	kcat/KM [mM/s]
4	Ki Value [mM]
13	KM Value [mM]
16	Localization
1	Metals/Ions
4	Molecular Weight [Da]
27	Natural Substrates/ Products (Substrates)
82	Organism
7	pH Optimum
3	Posttranslational Modification
8	Protein Variants
15	Purification (Commentary)
6	Reaction
38	Reference
2	Renatured (Commentary)
11	Source Tissue
8	Specific Activity [micromol/min/mg]
80	Substrates and Products (Substrate)
9	Subunits
53	Synonyms
5	Temperature Optimum [°C]
11	Turnover Number [1/s]

Engineering

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Engineering on EC 3.4.22.48 - staphopain

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PROTEIN VARIANTS

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

C237A

Staphylococcus aureus

-

inactive ScpA mutant

731911

C243A

Staphylococcus aureus

-

inactive SspB mutant

731911

C237A

Staphylococcus aureus AH1263

-

inactive ScpA mutant

-

C243A

Staphylococcus aureus AH1263

-

inactive SspB mutant

-

C21A

Staphylococcus warneri

-

purification of native staphopain B unsuccessful since strain SW035 produces only minimally detectable protease activity, active-site mutation generated by site-directed mutagenesis, catalytically inactive mutant StpBwar C21A used for interaction studies

683202

additional information

3 entries

additional information

Staphylococcus aureus

-

exchange of active site cysteine residue for an alanine, inactive mutant, fusion to N-terminal His-tag

643962

additional information

Staphylococcus aureus

-

construction of an inactive mutant, active site mutation of staphopain A results in abrogation of production of both co-expressed proteins

667487

additional information

Staphylococcus aureus

-

inactivation of gene sspA by transposon insertion exertes a polar effect on genes sspB and sspC expression, the inactivation of all three genes results in attenuation of virulence

667029