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3.4.22.48: staphopain

This is an abbreviated version!
For detailed information about staphopain, go to the full flat file.

Word Map on EC 3.4.22.48

Reaction

broad endopeptidase action on proteins including elastin, but rather limited hydrolysis of small-molecule substrates. Assays are conveniently made with hemoglobin, casein or Z-Phe-Arg-NHMec as substrate =

Synonyms

Ecp, extracellular cysteine protease, SCPA, Sscp A, Sscp B, SspB, SspB protein, staphopain, staphopain A, staphopain B, staphopain C, staphylopain

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.22 Cysteine endopeptidases
                3.4.22.48 staphopain

Cloned

Cloned on EC 3.4.22.48 - staphopain

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
co-expression of His-tagged staphopain A and its inhibitor staphostatin A in Escherichia coli strain BL21(DE3) in inclusion bodies, recombinant enzyme is toxic for Escherichia coli cells, therefore a co-expression with the enzyme inhibitor is required for recombinant enzyme production, method optimization
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cysteine protease staphopain B of Staphylococcus warneri transformed into Escherichia coli strain BL21(DE3)pLysS, expression failed, mutant C21A used for recombinant protein production, expression in Escherichia coli strain BL21(DE3)
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DNA and partial amino acid sequence determination and analysis
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expressed in Escherichia coli
expressed in Escherichia coli strain ER2566
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expression of large amounts of active wild-type enzyme in Escherichia coli is not possible, probably due to toxicity for the host
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expression of wild-type enzyme as fusion protein in Escherichia coli, hybrid construct with a N-terminal GST-moiety linked to the proenzyme via a thrombin cleavable linker
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overexpressed in Staphylococcus aureus, His-tagged version expressed in Escherichia coli
overexpression of the inactive mutant in Escherichia coli BL21(DE3) as His-tagged protein
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prostaphopain B, expression as GST-fusion protein in Escherichia coli strain BL21(DE3)
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ssp and scp operons, genomic organization of ssp and scp genes, overview
ssp and scp operons, genomic organization of ssp and scp genes, overview, overexpression of scp operon genes in Escherichia coli
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Staphylococcus aureus UAMS-1 and its isogenic sarA, agr, and sarA agr regulatory mutant. ScpA is higher in abundance in the sarA and sarA agr mutants than in strain UAMS-1. Analysis of time-dependent ScpA amount during strain cultivation.
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