3.4.22.47: gingipain K
This is an abbreviated version!
For detailed information about gingipain K, go to the full flat file.
Word Map on EC 3.4.22.47
-
3.4.22.47
-
gingivalis
-
porphyromonas
-
periodontal
-
gingipains
-
arg-gingipains
-
proteinases
-
arginine-specific
-
crevicular
-
keystone
-
hrgpa
-
subgingival
-
molecular biology
-
black-pigmented
-
gingivalis-induced
-
coaggregation
-
medicine
-
arg-specific
-
gingipain-deficient
- 3.4.22.47
- gingivalis
- porphyromonas
- periodontal
-
gingipains
- arg-gingipains
- proteinases
-
arginine-specific
-
crevicular
-
keystone
- hrgpa
-
subgingival
- molecular biology
-
black-pigmented
-
gingivalis-induced
-
coaggregation
- medicine
-
arg-specific
-
gingipain-deficient
Reaction
endopeptidase with strict specificity for lysyl bonds =
Synonyms
gingipain, gingipain K, HbR, K-gingipain, K-specific gingipain protease, KGP, Lys-gingipain, Lys-specific cysteine proteinase gingipain, lysine gingipain, lysine-sepcific cysteine protease, lysine-specific gingipain, lysine-specific gingipain K, lysine-specific gingipain protease, lysine-specific gingipain proteinase, lysine-specific proteinase, porphypain, PrtP proteinase
ECTree
3.4.22.47 gingipain KAdvanced search results
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results (Substrates Products
Substrates Products on EC 3.4.22.47 - gingipain K
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SUBSTRATE 
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
acetyl-lysine-4-nitroanilide + H2O
acetyl-lysine + 4-nitroaniline
-
-
-
-
?
acid-soluble human placental type I collagen + H2O
fragments of acid-soluble human placental type I collagen
-
-
-
-
?
adrenocorticotropic hormone fragment 11-24 + H2O
fragments of adrenocorticotropic hormone fragment 11-24
-
-
-
?
benzyloxycarbonyl-L-Lys-p-nitroanilide + H2O
benzyloxycarbonyl-L-Lys + p-nitroaniline + H2O
bovine serum albumin + H2O
fragments of bovine serum albumin
-
-
-
-
?
bradykinin + H2O
fragments of bradykinin
-
together with gingipain R, gingipain K induces vascular permeability enhancement in human plasma by cleaving bradykinin from high molecular weight kininogen
-
?
CD46 + H2O
?
-
recombinant CD46 is degraded by Lys-gingipain in a dose-dependent manner
-
-
?
elafin + H2O
?
all three gingipains have the ability to degrade elafin (endogenous inhibitor secreted by epithelial cells)
-
-
?
focal adhesion kinase + H2O
?
-
Kgp is responsible for degradation of integrin-related molecules
-
-
?
haptoglobin + H2O
fragments of haptoglobin
-
human serum haptoglobin
-
?
hemoglobin + H2O
fragments of hemoglobin
-
human serum hemoglobin
-
?
hemopexin + H2O
fragments of hemopexin
-
human serum hemopexin
-
?
IgG1 + H2O
?
-
The heavy chain of IgG1 is cleaved at a single site within the hinge region, generating Fab and Fc fragments
-
-
?
IgG3 + H2O
?
-
IgG3 is cleaved within the heavy chain and at several sites around the CH2 region
-
-
?
Met-Lys-bradykinin + H2O
fragments of Met-Lys-bradykinin
-
-
-
?
monocyte chemotactic protein 1 + H2O
fragments of monocyte chemotactic protein 1
-
degradation of human monocyte chemotactic protein 1 in Porphyromonas gingivalis-infected human umbilical vein endothelial cells
-
?
N-(p-tosyl)-Gly-Pro-Lys-4-nitroanilide + H2O
N-(p-tosyl)-Gly-Pro-Lys + 4-nitroaniline
-
-
-
-
?
N-4-tosyl-Gly-Pro-Lys-4-nitroanilide + H2O
N-4-tosyl-Gly-Pro-Lys + 4-nitroaniline
-
-
-
-
?
N-alpha-acetyl-L-lysine-4-nitroanilide + H2O
N-alpha-acetyl-L-lysine + 4-nitroaniline
-
-
-
-
?
N-alpha-acetyl-L-lysine-p-nitroanilide + H2O
N-alpha-acetyl-L-lysine + 4-nitroaniline
-
-
-
-
r
N-alpha-benzoyl-DL-lysine 4-nitroanilide + H2O
N-alpha-benzoyl-DL-lysine + 4-nitroaniline
-
-
-
-
?
N-alpha-benzyloxycarbonyl-L-lysine-4-nitroanilide + H2O
N-alpha-benzyloxycarbonyl-L-lysine + 4-nitroaniline
N-p-tosyl-Gly-Pro-Lys-4-nitroanilide + H2O
N-p-tosyl-Gly-Pro-Lys + 4-nitroaniline
-
-
-
-
?
Nalpha-acetyl-L-lysine 4-nitroanilide + H2O
Nalpha-acetyl-L-lysine + 4-nitroaniline
-
-
-
-
?
Nalpha-benzyloxycarbonyl-L-lysine 4-nitroanilide + H2O
Nalpha-benzyloxycarbonyl-L-lysine + 4-nitroaniline
-
-
-
-
?
paxillin + H2O
?
-
Kgp is responsible for degradation of integrin-related molecules
-
-
?
protease-activated receptor-2 + H2O
?
-
specific substrate for Lys-gingipain
-
-
?
t-butyl-oxycarbonyl-L-Glu-L-Lys-L-Lys-4-methyl-7-coumarylamide + H2O
t-butyl-oxycarbonyl-L-Glu-L-Lys-L-Lys + 7-amino-4-methylcoumarin
-
41% of activity with t-butyl-oxycarbonyl-L-Val-L-Leu-L-Lys-4-methyl-7-coumarylamide
-
?
t-butyl-oxycarbonyl-L-Val-L-Leu-L-Lys-4-methyl-7-coumarylamide + H2O
t-butyl-oxycarbonyl-L-Val-L-Leu-L-Lys + 7-amino-4-methylcoumarin
-
gingipain K has a stron preference for substrates containing Lys in the p1 site
-
?
t-butyloxycarbonyl-Val-Leu-Lys-Phe-Arg-4-methyl-7-coumarylamide
t-butyl-oxycarbonyl-Val-Leu-Lys + Phe-Arg-4-methyl-7-coumarylaminde
-
-
-
-
?
thrombomodulin + H2O
?
-
Lys-gingipain and Arg-gingipain cleave thrombomodulin in vitro
-
-
?
transferrin + H2O
fragments of transferrin
-
human serum transferrin
-
?
Val-Leu-Lys-4-nitroanilide + h2O
Val-Leu-Lys + 4-nitroaniline
-
-
-
-
?
benzyloxycarbonyl-L-Lys-p-nitroanilide + H2O
benzyloxycarbonyl-L-Lys + p-nitroaniline + H2O
-
-
-
?
benzyloxycarbonyl-L-Lys-p-nitroanilide + H2O
benzyloxycarbonyl-L-Lys + p-nitroaniline + H2O
-
7% of activity with N-p-tosyl-Gly-Pro-Lys-p-nitroanilide
-
-
?
benzyloxycarbonyl-L-Lys-p-nitroanilide + H2O
benzyloxycarbonyl-L-Lys + p-nitroaniline + H2O
-
-
-
?
D-Val-Leu-Lys-p-nitroanilide + H2O
D-Val-Leu-Lys + p-nitroaniline
-
-
-
?
D-Val-Leu-Lys-p-nitroanilide + H2O
D-Val-Leu-Lys + p-nitroaniline
-
-
-
?
D-Val-Leu-Lys-p-nitroanilide + H2O
D-Val-Leu-Lys + p-nitroaniline
-
-
-
?
D-Val-Phe-Lys-p-nitroanilide + H2O
D-Val-Phe-Lys + p-nitroaniline
-
-
-
?
D-Val-Phe-Lys-p-nitroanilide + H2O
D-Val-Phe-Lys + p-nitroaniline
-
70% of activity with N-p-tosyl-Gly-Pro-Lys-p-nitroanilide
-
?
D-Val-Phe-Lys-p-nitroanilide + H2O
D-Val-Phe-Lys + p-nitroaniline
-
-
-
?
Fibrinogen + H2O
Fragments of fibrinogen
-
prominent target among plasma proteins
-
?
Fibrinogen + H2O
Fragments of fibrinogen
-
most prominent target among plasma proteins
-
-
?
Hemoglobin + H2O
?
-
degradation, the enzyme forms a complex with the outer membrane receptor HmuR required for binding and utilization of hemoglobin and hemin, overview
-
-
?
interleukin 8 + H2O
fragments of interleukin 8
-
degradation of human interleukin 8 in Porphyromonas gingivalis-infected human umbilical vein endothelial cells
-
?
interleukin-1beta + H2O
?
-
biological inactivation and degradation, low activity, cytokine degradation is mainly the result of Lys-gingipain
-
-
?
interleukin-6 + H2O
?
-
biological inactivation and degradation
-
-
?
interleukin-8 + H2O
?
-
biological inactivation and degradation
-
-
?
methemoglobin + H2O
?
-
methemoglobin, formed by treatment of oxyHb with either NaNO2 or by pre-incubation with HRgpA, is rapidly degraded by Kgp compared to oxyhemoglobin
-
-
?
methemoglobin + H2O
?
-
degradation, ligation of methemoglobin with N3-, which effectively blocks heme dissociation from the protein, prevents hemoglobin breakdown
-
-
?
methemoglobin + H2O
?
-
from horse, degradation, no activity with oxyhemoglobin
-
-
?
methemoglobin + H2O
?
-
methemoglobin, formed by treatment of oxyHb with either NaNO2 or by pre-incubation with HRgpA, is rapidly degraded by Kgp compared to oxyhemoglobin
-
-
?
N-alpha-benzyloxycarbonyl-L-lysine-4-nitroanilide + H2O
N-alpha-benzyloxycarbonyl-L-lysine + 4-nitroaniline
-
-
-
-
?
N-alpha-benzyloxycarbonyl-L-lysine-4-nitroanilide + H2O
N-alpha-benzyloxycarbonyl-L-lysine + 4-nitroaniline
-
-
-
-
?
N-p-tosyl-Gly-Pro-Lys-p-nitroanilide + H2O
N-p-tosyl-Gly-Pro-Lys + p-nitroaniline
-
-
-
?
N-p-tosyl-Gly-Pro-Lys-p-nitroanilide + H2O
N-p-tosyl-Gly-Pro-Lys + p-nitroaniline
-
-
-
?
N-p-tosyl-Gly-Pro-Lys-p-nitroanilide + H2O
N-p-tosyl-Gly-Pro-Lys + p-nitroaniline
-
gingipain K cleaves specifically on the C-terminal side of peptide substrates
-
?
N-p-tosyl-Gly-Pro-Lys-p-nitroanilide + H2O
N-p-tosyl-Gly-Pro-Lys + p-nitroaniline
-
gingipain K cleaves specifically on the C-terminal side of peptide substrates
-
?
neurotensin + H2O
pGlu-Leu-Tyr-Glu-Asn-Lys + Pro-Arg-Arg-Pro-Tyr-Ile-Leu
-
-
-
?
neurotensin + H2O
pGlu-Leu-Tyr-Glu-Asn-Lys + Pro-Arg-Arg-Pro-Tyr-Ile-Leu
-
-
-
?
osteoprotegerin + H2O
?
-
digestion, enhanced osteoclastogenesis by Kgp, in co-cultures of mouse bone-marrow cells and osteoblasts, formation of multinucleated osteoclasts induced by 1alpha,25-dihydroxyvitamin D3 is augmented by Kgp. The specific enzymatic activity of Kgp is necessary for osteoclast formation, overview
-
-
?
osteoprotegerin + H2O
?
-
digestion, enhanced osteoclastogenesis by Kgp, in co-cultures of mouse bone-marrow cells and osteoblasts, formation of multinucleated osteoclasts induced by 1alpha,25-dihydroxyvitamin D3 is augmented by Kgp. The specific enzymatic activity of Kgp is necessary for osteoclast formation, overview
-
-
?
TNFalpha + H2O
?
-
degradation, leading to inhibition of biological functions of TNFalpha, overview
-
-
?
additional information
?
-
-
a combination of both arginine- and lysine-specific gingipain activity is necessary for the generation of the micro-oxo bishaem-containing pigment from haemoglobin, interaction with oxyhemoglobin, overview
-
-
?
additional information
?
-
-
gingipain K adds to gingipain R-mediated coaggregation of Porphyromonas gingivalis with other oral bacteria, it is a virulence factor, overview
-
-
?
additional information
?
-
-
gingipains are essential for bacterial virulence and survival
-
-
?
additional information
?
-
-
the C-terminal domains of the gingipain K polyprotein are necessary for assembly of the active enzyme and expression of associated activities
-
-
?
additional information
?
-
-
the enzyme degrades host iron- and heme-containing proteins, regulation of enzyme expression, overview, inhibition of gingipain increases the hmuR gene expression encoding the heme/hemoglobin receptor HmuR, and decreases the cell growth in the early and middle stages, but not in the late stages, hmuR expression inhibition decreases enzyme expression
-
-
?
additional information
?
-
-
the enzyme inactivates a cell surface ligand on Porphyromonas gingivalis that induces TLR2-and TLR4-independent signaling involving CD25, but has no effect on TLR2-and TLR4-dependent signaling, overview
-
-
?
additional information
?
-
-
role of the Sn binding pocket, molecular basis for substrate specificity, overview
-
-
?
additional information
?
-
-
extracellular gingipain protease activities cause a lack of secondary cytokine response in human cells after challenge with live Porphyromonas gingivalis
-
-
?
additional information
?
-
-
gingipains are considered to be key virulence factors of the bacterium in relation to periodontal diseases. Kgp suppresses interleukin-8 production in human HSC-2 epithelial cells requiring both the hemagglutinin and the enzymic domains. Suppression of IL-8 resultes in attenuation of the cellular recognition of bacteria, and as a consequence, sustain chronic inflammation
-
-
?
additional information
?
-
-
gingipains are cysteine proteinases and virulence factors of Porphyromonas gingivalis, the major causative bacterium of periodontal disease. Gingipains stimulate interleukin-8 secretion from human THP-1 cells, which is completely inhibited by proteinase inhibitors of gingipain and is increased in the presence of pathogen-associated molecular patterns, PAMPs. The synergism of gingipain HRgpA and PAMPs stimulates interleukin IL-6, and monocyte chemoattractant protein MCP-1 secretion, overview
-
-
?
additional information
?
-
-
gingipains are key virulence determinants of Porphyromonas gingivalis and play a crucial role in pathogenicity
-
-
?
additional information
?
-
-
gingipains reduce cyclin expression and cause early G1 arrest, leading to the inhibition of cellular proliferation in murine ST2 osteoblastic/stromal cells, overview
-
-
?
additional information
?
-
-
the membrane protein PG27, encoded by gene PG0027, is essential for the secretion of gingipains, overview
-
-
?
additional information
?
-
-
gingipains reduce cyclin expression and cause early G1 arrest, leading to the inhibition of cellular proliferation in murine ST2 osteoblastic/stromal cells, overview
-
-
?
additional information
?
-
-
gingipains are essential for bacterial virulence and survival
-
-
?
additional information
?
-
-
role of the Sn binding pocket, molecular basis for substrate specificity, overview
-
-
?
additional information
?
-
-
gingipains are considered to be key virulence factors of the bacterium in relation to periodontal diseases. Kgp suppresses interleukin-8 production in human HSC-2 epithelial cells requiring both the hemagglutinin and the enzymic domains. Suppression of IL-8 resultes in attenuation of the cellular recognition of bacteria, and as a consequence, sustain chronic inflammation
-
-
?
additional information
?
-
-
the membrane protein PG27, encoded by gene PG0027, is essential for the secretion of gingipains, overview
-
-
?
