3.4.22.47: gingipain K

This is an abbreviated version!
For detailed information about gingipain K, go to the full flat file.

Reaction

endopeptidase with strict specificity for lysyl bonds =

Synonyms

gingipain, gingipain K, HbR, K-gingipain, K-specific gingipain protease, KGP, Lys-gingipain, Lys-specific cysteine proteinase gingipain, lysine gingipain, lysine-sepcific cysteine protease, lysine-specific gingipain, lysine-specific gingipain K, lysine-specific gingipain protease, lysine-specific gingipain proteinase, lysine-specific proteinase, porphypain, PrtP proteinase

ECTree

     3 Hydrolases

         3.4 Acting on peptide bonds (peptidases)

             3.4.22 Cysteine endopeptidases

                3.4.22.47 gingipain K

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Results	in table
15	Activating Compound
59	AA Sequence
7	Application
1	CAS Registry Number
5	Cloned(Commentary)
3	Crystallization (Commentary)
14	General Information
1	General Stability
53	Inhibitors
3	Ki Value [mM]
7	KM Value [mM]
14	Localization
1	Metals/Ions
10	Molecular Weight [Da]
33	Natural Substrates/ Products (Substrates)
126	Organism
7	PDB ID
21	pH Optimum
1	pH Range
3	pH Stability
4	Posttranslational Modification
18	Protein Variants
11	Purification (Commentary)
3	Reaction
1	Reaction Type
65	Reference
6	Source Tissue
3	Specific Activity [micromol/min/mg]
105	Substrates and Products (Substrate)
15	Subunits
93	Synonyms
19	Temperature Optimum [°C]
6	Temperature Stability [°C]
1	Turnover Number [1/s]

Purification

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Purification on EC 3.4.22.47 - gingipain K

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PURIFICATION (Commentary)

ORGANISM

UNIPROT

LITERATURE

-

Porphyromonas gingivalis

-

638852

acetone precipitate, Sephadex G-150, arginine-Sepharose

Porphyromonas gingivalis

-

638843

ammonium sulfate, immunoaffinity column, Mono S

Porphyromonas gingivalis

-

638848

ammonium sulfate, Mono Q, chromatofocusing on MonoP

Porphyromonas gingivalis

-

30352

Lys-gingipain is purified from the cell envelope extract of Porphyromonas gingivalis KDP112 by affinity chromatography on arginine Sepharose 4B

Porphyromonas gingivalis

-

710175

native extracellular enzyme from culture medium

Porphyromonas gingivalis

-

669799

native Kgp from culture supernatant

Porphyromonas gingivalis

-

699480

native Lys-gingipain from cell culture supernatant, re-activation of the proteases by reducing the active-centre thiol group partially oxidized during the purification process

Porphyromonas gingivalis

-

696111

purified by using gel-filtration and arginine-Sepharose chromatography

Porphyromonas gingivalis

-

681787

using gel-filtration

Porphyromonas gingivalis

-

677827

using Ni-NTA chromatography and gel filtration

Porphyromonas gingivalis

-

710002