3.4.22.45: helper-component proteinase
This is an abbreviated version!
For detailed information about helper-component proteinase, go to the full flat file.
Word Map on EC 3.4.22.45
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3.4.22.45
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viruses
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potato
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nicotiana
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potyviruses
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polyproteins
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benthamiana
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potyviral
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aphid
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potyviridae
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mottle
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etch
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turnip
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cistron
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plum
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zucchini
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ringspot
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papaya
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nia-pro
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cucurbit
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tritimovirus
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non-persistent
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ipomovirus
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pvyntn
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genome-linked
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rna-silencing
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veinal
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feathery
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agriculture
- 3.4.22.45
- viruses
- potato
- nicotiana
- potyviruses
- polyproteins
- benthamiana
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potyviral
- aphid
- potyviridae
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mottle
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etch
- turnip
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cistron
- plum
- zucchini
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ringspot
- papaya
- nia-pro
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cucurbit
- tritimovirus
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non-persistent
- ipomovirus
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pvyntn
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genome-linked
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rna-silencing
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veinal
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feathery
- agriculture
Reaction
hydrolyses a Gly-/-Gly bond at its own C-terminus, commonly in the sequence -Tyr-Xaa-Val-Gly-/-Gly, in the processing of the potyviral polyprotein =
Synonyms
HC-Pro, HC-Pro protein, HC-Pro proteinase, HcPro, helper component protease, helper component proteinase, helper component-protease, helper component-proteinase, helper-component proteinase, More, potyvirus helper component proteinase, PVA HC-Pro, strong silencing suppressor P1
ECTree
Advanced search results
Engineering
Engineering on EC 3.4.22.45 - helper-component proteinase
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D193Y
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mutants of helper component protease HC-Pro of clover yellow vein virus, generated by site-directed mutagenesis or by PCR-based primer extension mutagenesis, revertant of D193Y also created, single mutant alone does not induce lethal necrosis
I33
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mutants of helper component protease HC-Pro of clover yellow vein virus generated by site-directed mutagenesis or by PCR-based primer extension mutagenesis
R51I
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mutants of helper component protease HC-Pro of clover yellow vein virus generated by site-directed mutagenesis or by PCR-based primer extension mutagenesis
T27I
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mutants of helper component protease HC-Pro of clover yellow vein virus generated by site-directed mutagenesis or by PCR-based primer extension mutagenesis, revertant of T27I also created, single mutant alone does not induce lethal necrosis but retains ability to induce necrotic symptoms
P310A/T311A
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the mutations disrupt interaction between the enzyme and capsid protein
D446A
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mutation does not abolish in vitro interaction between helper-component-proteinase and coat protein
E450A
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mutation does not abolish in vitro interaction between helper-component-proteinase and coat protein
H429A
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mutation does not abolish in vitro interaction between helper-component-proteinase and coat protein
K432A
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mutation does not abolish in vitro interaction between helper-component-proteinase and coat protein
K452A
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mutation does not abolish in vitro interaction between helper-component-proteinase and coat protein
R455A
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mutation abolishes in vitro interaction between helper-component-proteinase and coat protein
T310A
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mutation abolishes in vitro interaction between helper-component-proteinase and coat protein
A387E
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no RNA silencing suppressor activity, Nicotiana benthamiana plant inoculated with infectious transcript shows no etching
C390W
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no RNA silencing suppressor activity, Nicotiana benthamiana plant inoculated with infectious transcript shows no etching
C694S
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mutant is proteolytically active and capable of cell-to-cell and long-distance movements
D421N
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no RNA silencing suppressor activity, Nicotiana benthamiana plant inoculated with infectious transcript shows no etching
D715E
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mutant is proteolytically active and capable of cell-to-cell and long-distance movements
E273A
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no RNA silencing suppressor activity, Nicotiana benthamiana plant inoculated with infectious transcript shows no etching
E299A/D300A
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RNA silencing suppressor activity reduced compared to wild-type, Nicotiana benthamiana plant inoculated with infectious transcript shows no etching
E360A/D361A
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RNA silencing suppressor activity reduced compared to wild-type, Nicotiana benthamiana plant inoculated with infectious transcript shows mild etching
E443K
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RNA silencing suppressor activity increased compared to wild-type, Nicotiana benthamiana plant inoculated with infectious transcript shows etching
E452D
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RNA silencing suppressor activity similar to wild-type, Nicotiana benthamiana plant inoculated with infectious transcript shows no etching
I11L
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RNA silencing suppressor activity increased compared to wild-type, Nicotiana benthamiana plant inoculated with infectious transcript shows etching
I442M
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no RNA silencing suppressor activity, Nicotiana benthamiana plant inoculated with infectious transcript shows no etching
K309N
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RNA silencing suppressor activity similar to wild-type, Nicotiana benthamiana plant inoculated with infectious transcript shows etching
K454T
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RNA silencing suppressor activity increased compared to wild-type, Nicotiana benthamiana plant inoculated with infectious transcript shows etching
N193Y
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RNA silencing suppressor activity similar to wild-type, Nicotiana benthamiana plant inoculated with infectious transcript shows no etching
N194D
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RNA silencing suppressor activity similar to wild-type, Nicotiana benthamiana plant inoculated with infectious transcript shows no etching
N200S
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RNA silencing suppressor activity increased compared to wild-type, Nicotiana benthamiana plant inoculated with infectious transcript shows etching
P311L
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RNA silencing suppressor activity similar to wild-type, Nicotiana benthamiana plant inoculated with infectious transcript shows etching
Q265H
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RNA silencing suppressor activity similar to wild-type, Nicotiana benthamiana plant inoculated with infectious transcript shows etching
R127G
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no RNA silencing suppressor activity, Nicotiana benthamiana plant inoculated with infectious transcript shows no etching
R165G
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RNA silencing suppressor activity similar to wild-type, Nicotiana benthamiana plant inoculated with infectious transcript shows etching
R240A/K241A/H242A
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no RNA silencing suppressor activity, Nicotiana benthamiana plant inoculated with infectious transcript shows no etching
R247A/K248A
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no RNA silencing suppressor activity, Nicotiana benthamiana plant inoculated with infectious transcript shows no etching
S610T
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mutant is proteolytically active and capable of cell-to-cell and long-distance movements
V135A
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no RNA silencing suppressor activity, Nicotiana benthamiana plant inoculated with infectious transcript shows no etching
V192A
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RNA silencing suppressor activity reduced compared to wild-type, Nicotiana benthamiana plant inoculated with infectious transcript shows mild etching
V355L
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RNA silencing suppressor activity similar to wild-type, Nicotiana benthamiana plant inoculated with infectious transcript shows etching
V419A
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RNA silencing suppressor activity similar to wild-type, Nicotiana benthamiana plant inoculated with infectious transcript shows etching
Y234H
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RNA silencing suppressor activity similar to wild-type, Nicotiana benthamiana plant inoculated with infectious transcript shows etching
Y344S
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RNA silencing suppressor activity similar to wild-type, Nicotiana benthamiana plant inoculated with infectious transcript shows mild etching
Y423H
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RNA silencing suppressor activity increased compared to wild-type, Nicotiana benthamiana plant inoculated with infectious transcript shows etching
C16A
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substitution mutants introduced into amino-proximal region of helper component protease HC-Pro, vector transmission abolished
D506Y
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very mild symptoms of zucchini yellow mosaic virus infection
R180I/E396N
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induces transient leaf mottling, affects symptom severity and viral pathogenicity
R180I/F205L/E396N
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the mutant is completely defective in its capacity to block miRNA regulation
additional information
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inactivation by point mutation HCL134H, inhibits synergism and RNA silencing activity
additional information
random-insertion scanning mutagenesis, generation of an pentapeptide-insertion scanning mutagenesis library of helper component protease HC-Pro, performed by using the mutation generation system F701 MGS, pentapeptide scanning mutants in pBIN61S-HC-Pro mapped by restriction digestion, insertion position determined by sequencing
additional information
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random-insertion scanning mutagenesis, generation of an pentapeptide-insertion scanning mutagenesis library of helper component protease HC-Pro, performed by using the mutation generation system F701 MGS, pentapeptide scanning mutants in pBIN61S-HC-Pro mapped by restriction digestion, insertion position determined by sequencing
additional information
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random-insertion scanning mutagenesis, generation of an pentapeptide-insertion scanning mutagenesis library of helper component protease HC-Pro, performed by using the mutation generation system F701 MGS, pentapeptide scanning mutants in pBIN61S-HC-Pro mapped by restriction digestion, insertion position determined by sequencing
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additional information
mutations in the 4E binding site of HCpro reducing virulence of PVA
additional information
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mutations in the 4E binding site of HCpro reducing virulence of PVA
additional information
certain mutations introduced to the short, variable region of the enzyme increase accumulation of the enzyme and prolong the time which it remains effective in suppression of RNA silencing
additional information
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certain mutations introduced to the short, variable region of the enzyme increase accumulation of the enzyme and prolong the time which it remains effective in suppression of RNA silencing
additional information
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mutations in the 4E binding site of HCpro reducing virulence of PVA
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additional information
three deletion mutants designed for HC-Pro protein of potato virus Y, HC-Pro1 (residues 98 to 456), HC-Pro2 (residues 1 to 298) and HC-Pro3 (residues 1 to 97) used for yeast two-hybrid analysis
additional information
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three deletion mutants designed for HC-Pro protein of potato virus Y, HC-Pro1 (residues 98 to 456), HC-Pro2 (residues 1 to 298) and HC-Pro3 (residues 1 to 97) used for yeast two-hybrid analysis
additional information
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mutations of a short variable region affect interactions with a microtubule-associated protein and induce necrotic responses in tobacco leaves
additional information
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three deletion mutants designed for HC-Pro protein of potato virus Y, HC-Pro1 (residues 98 to 456), HC-Pro2 (residues 1 to 298) and HC-Pro3 (residues 1 to 97) used for yeast two-hybrid analysis
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additional information
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mutations of a short variable region affect interactions with a microtubule-associated protein and induce necrotic responses in tobacco leaves
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additional information
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mutants used in compensatory evolution experiments: E299A (0.6127 RNA-silencing suppression activity relative to wild type, numeration corresponds to the location in the full polyprotein), E360A/D361A (0.1507 RNA-silencing suppression activity relative to wild type, numeration corresponds to the location in the full polyprotein), V192A (0.7388 RNA-silencing suppression activity relative to wild type, numeration corresponds to the location in the full polyprotein), Y423H (1.1020 RNA-silencing suppression activity relative to wild type, numeration corresponds to the location in the full polyprotein), E443K (1.2631 RNA-silencing suppression activity relative to wild type, numeration corresponds to the location in the full polyprotein), K454T (1.1592 RNA-silencing suppression activity relative to wild type, numeration corresponds to the location in the full polyprotein), mutations fixed during compensatory evolution experiments are also characterized by RNA-silencing suppression activity values
additional information
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mutations of a short variable region affect interactions with a microtubule-associated protein and induce necrotic responses in tobacco leaves
additional information
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mutations of a short variable region affect interactions with a microtubule-associated protein and induce necrotic responses in tobacco leaves
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additional information
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construction of deletion mutants of the enzyme with 5'-proximal deletions of 12 to 720 nucleotides, mutant infection of wheat analysis, overview
additional information
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mutant generation within coding region of helper component protease HC-Pro protein, nine synonymous substitutions and 15 of 25 non-synonymous substitutions shown to be without phenotypic effect, four non-synonymous substitutions, including one reverting to wild type, shown to disply attenuated systemic infection, six non-synonymous substitutions and one nonsense substitution shown to display abolished systemic infectivity
additional information
a point mutation in the highly conserved FRNK box produces the HC-ProFINK protein that shows no sRNA binding
additional information
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a point mutation in the highly conserved FRNK box produces the HC-ProFINK protein that shows no sRNA binding
additional information
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deletion of the first 93 residues of the N-terminus of ZYMV HC-Pro. Mutation of the conserved Gly456 residue does not affect the autoproteolytic activity of ZYMV HC-Pro
additional information
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generation of different N- and C-terminal truncated DELTA HC-Pro constructs, i.e. comprising residues 93-456, 114-456, and 139-456, or 1-322, 1-350, and 1-372, construction of recombinant mutant MBP:HA-HC-ProFRNK, MBP:HAHC-ProFINK, and truncated MBP:HA-HC-ProFRNK. HC-ProFRNK/FINK clearly inhibits Arabidopsis thaliana HEN1 activity in vitro. HC-ProFRNK/FINK, but not the truncated proteins HC-Pro 93-456, and HC-Pro 1-372 displaying decreased in vitro affinity for AtHEN1 binding, inhibits the methyltransferase activity of AtHEN1 in vitro