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3.4.22.45: helper-component proteinase

This is an abbreviated version!
For detailed information about helper-component proteinase, go to the full flat file.

Word Map on EC 3.4.22.45

Reaction

hydrolyses a Gly-/-Gly bond at its own C-terminus, commonly in the sequence -Tyr-Xaa-Val-Gly-/-Gly, in the processing of the potyviral polyprotein =

Synonyms

HC-Pro, HC-Pro protein, HC-Pro proteinase, HcPro, helper component protease, helper component proteinase, helper component-protease, helper component-proteinase, helper-component proteinase, More, potyvirus helper component proteinase, PVA HC-Pro, strong silencing suppressor P1

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.22 Cysteine endopeptidases
                3.4.22.45 helper-component proteinase

Cloned

Cloned on EC 3.4.22.45 - helper-component proteinase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
14 isolates of turnip mosaic virus, RNA extraction, reverse transctiption, amplification using RT-PCR, cloned into pGEM-T vector for sequence analysis and phylogenetic analysis. HC-Pro genes comprise 1374 nucleotides encoding a polypeptide of 458 amino acids
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42 isolates of plum pox virus (different types of strains. plum pox virus-D, plum pox virus-M, plum pox virus-Rec). Single-strand conformation polymorphism analysis and low-stringency single specific primer PCR analysis are performed on the HC-Pro region of the genome for genotyping
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as P1/HC-Pro, expressed in Nicotiana benthamiana
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binary constructs for overexpression of helper component proteinase HC-Pro in Tobacco etch virus generated, overexpression in Nicotina benthamiana via infiltration with Agrobacterium tumefaciens shown, binary vector pBin19-GFP used, pHannibal vector used for GFP-silencing inverted-repeat constructs
expressed in Arabidopsis
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expressed in Escherichia coli
expressed in Escherichia coli and Nicotiana tabacum
expressed in Escherichia coli JM109 (DE3) cells
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expressed in Escherichia coli, transformation into Agrobacterium tumefaciens C58C1 by using the binary plant expression vector pBIN61Sa
expressed in Nicotiana benthamiana
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expression in immature Glycine max cotyledonary explants, co-transfection and co-culture with Agrobacterium tumefaciens strain KYRT1 transfected with a hygromycin phosphotransferase gene, the enzyme enhances recorvery of somatic embryos of Glycine max, effects on gene expression, overview
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expression of HCPro with YN fused to the HCpro N terminus in Nicotiana benthamiana, and translation initiation factors eIF(iso)4E (Tiso4Eb and Piso4Eb) and eIF4E (T4Ea and P4Eb) of tobacco (T) and potato (P) with YC fused to the N- or C-terminus. Leaves are infiltrated with pairs of Agrobacterium strains expressing tester proteins tagged with the opposite halves of YFP
expression of HCPro with YN fused to the HCpro N terminus in Nicotiana benthamiana, and translation initiation factors eIF(iso)4E (Tiso4Eb and Piso4Eb) and eIF4E (T4Ea and P4Eb) of tobacco (T) and potato (P) with YC fused to the N- or C-terminus. Leaves are infiltrated with pairs of Agrobacterium strains expressing tester proteins tagged with the opposite halves of YFP. Interactions are negative in the YTHS, an artificial system in which protein interactions are tested in the nucleus
expression of His6-tagged or MBP-fused wild-type enzyme and of MBP-fused mutant HC-ProFINK protein in Escherichia coli strain BL21 (DE3)
expression of recombinant His-tagged or Strep-tagged enzyme in LMV in plant leaves
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expression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)
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fragment of pS81-SA containing the HC-Pro protein coding region used for generation of substitution mutations, ligation into pGEM5zf+ and transformation into Escherichia coli DH5a, 250 PCR-generated clones screened for mutations by single-strand conformation polymorphism SSCP analysis
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full-length coding sequence of HC-Pro of potato virus Y fused to the GAL4 DNA-binding domain, cloned into pGBKT7 via BamHI/PstI digestion to form pGBKT7-HC-Pro, different plasmid constructs generated, overview of domains and deletion mutants of HC-Pro given, transformation into Saccharomyces cerevisiae after subcloning into pGBKT7
functional expression of wild-type MBP:HA-HC-Pro:GFP and truncated mutant MBP:HA-HC-Pro:GFP N1, i.e. enzyme tagged with HA, GFP, and maltose-binding protein, in Escherichia coli leads to autoproteolytic cleavage of the green fluorescent protein, GFP
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genetic organization, enzyme replacements, overview
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HC-Pro is indispensable for Papaya ringspot virus infection in zucchini, N-terminus of HC-Pro is involved in systemic infection of PRSV
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HC-Pro is involved in virulence on Rsv1-genotype soybean (Rsv1 is a single dominant resistance gene), influence of single-point mutations on virulence analyzed
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HC-Pro suppresses the RNA silencing induced by sense RNA and dsRNA
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in vitro translation of transcripts in rabbit reticulocyte lysate or wheat germ extract, expression in Escherichia coli as His-tagged protein
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mutations in HC-Pro for analysing virulence on soybean, HC-Pro complementation of viral protein P3 is essential for virulence on Rsv1-genotype soybean (Rsv1 is a single dominant resistance gene)
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nucleotide and amino acid sequence determination, coding region of 1152 nucleotides, expression of wild-type and deletion mutants in recombinant Wheat streak mosaic virus in wheat
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plasmid pTEV7DA with an infectious TEV clone used as source of wild-type virus and template for site-directed mutagenesis. Wild-type and mutants are cloned into pBIN61 vector, electroporation of Agrobacterium tumefaciens for transient expression assays and quantification of suppression activity.
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recombinant expression using Potato virus X, PVX, in Nicotiana benthamiana, expression induces the pathogenicity of the Potato virus X manifesting a necrosis in plant and plant death
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small and large scale functional expression in Pichia pastoris strain GS115 of the gene fused into the Saccharomyces cerevisiae alpha-mating factor secretory peptide coding region, plant transfection using the Myus persicae aphid vector
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subcloned into pALTER vector for generation of substitution mutations by site-directed mutagenesis
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used in yeast two-hybrid assay
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used in yeast two-hybrid system, expressed in Escherichia coli, expressed in onion tissue (fusion protein)
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wild-type and mutant protein, expressed in Escherichia coli
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