3.4.22.44: nuclear-inclusion-a endopeptidase

This is an abbreviated version!
For detailed information about nuclear-inclusion-a endopeptidase, go to the full flat file.

Word Map on EC 3.4.22.44

3.4.22.44

polyproteins

etch

potyviruses

hc-pro

potyviridae

turnip

plum

cistron

genome-linked

nib-cp

trimv

agriculture

ringspot

poacevirus

niapro

Reaction

Hydrolyses glutaminyl bonds, and activity is further restricted by preferences for the amino acids in P6 - P1' that vary with the species of potyvirus, e.g. Glu-Xaa-Xaa-Tyr-Xaa-Gln-/-(Ser or Gly) for the enzyme from tobacco etch virus. The natural substrate is the viral polyprotein, but other proteins and oligopeptides containing the appropriate consensus sequence are also cleaved. =

Synonyms

49 kDa proteinase, 49 kDa-Pro, NIA, NIa protease, NIa protein, NIa proteins, NIa-Pro, nuclear inclusion a protease, nuclear inclusion a-protease, nuclear inclusion body protein a, nuclear inclusion protein A protease, nuclear inclusion protein-a protease, nuclear inclusion-a protease, potyviral nuclear inclusion a, potyvirus NIa protease, protease NIa, proteinase Nia, proteinase, polyprotein-processing, Nia, proteins (specific proteins and subclasses), NIa (nuclear inclusion, a), proteins NIa, proteins, small nuclear inclusion NIa, tobacco vein mottling virus protease

ECTree

3 Hydrolases

3.4 Acting on peptide bonds (peptidases)

3.4.22 Cysteine endopeptidases

3.4.22.44 nuclear-inclusion-a endopeptidase

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Results	in table
74	AA Sequence
7	Application
1	CAS Registry Number
18	Cloned(Commentary)
1	Crystallization (Commentary)
10	General Information
2	Inhibitors
2	kcat/KM [mM/s]
22	KM Value [mM]
2	Localization
3	Molecular Weight [Da]
23	Natural Substrates/ Products (Substrates)
67	Organism
4	PDB ID
3	pH Optimum
1	Posttranslational Modification
14	Protein Variants
7	Purification (Commentary)
1	Reaction
1	Reaction Type
47	Reference
1	Specific Activity [micromol/min/mg]
44	Substrates and Products (Substrate)
3	Subunits
34	Synonyms
5	Temperature Optimum [°C]
1	Temperature Stability [°C]
22	Turnover Number [1/s]

KM Value

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KM Value on EC 3.4.22.44 - nuclear-inclusion-a endopeptidase

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0.217

acetyl-Arg-Glu-Thr-Val-Arg-Phe-Gln-Ser-Asp-amide

tobacco vein mottling virus

137278

0.216

acetyl-Asn-Asn-Val-Arg-Phe-Gln-Ser-Leu-amide

tobacco vein mottling virus

137278

0.51 - 2.83

acetyl-Glu-Pro-Thr-Val-Tyr-His-Gln-Thr-Leu-amide

8 entries

0.069 - 0.271

Pro-Thr-Thr-Glu-Leu-Tyr-Phe-Gln-Ser-Gly-Thr-Val-Asp-Arg-Arg

2 entries

0.082 - 0.108

TETVRFQSGTRR

2 entries

0.05 - 11.4

WDGGEVAHQAGESV

8 entries