3.4.22.33: Fruit bromelain
This is an abbreviated version!
For detailed information about Fruit bromelain, go to the full flat file.
Reaction
Hydrolysis of proteins with broad specificity for peptide bonds. Bz-Phe-Val-Arg-/-NHMec is a good synthetic substrate, but there is no action on Z-Arg-Arg-NHMec (c.f. stem bromelain)
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Synonyms
ACMD2_17643, Ananase, Bromelain, bromelain A, bromelain B, Bromelain, juice, Bromelase, Bromelin, EC 3.4.22.4, EC 3.4.22.5, EC 3.4.4.24, Extranase, fastuosain, FBM, Fruit bromelain, Fruit bromelain FA2, Juice bromelain, More, Pinase, Traumanase
ECTree
pH Optimum
pH Optimum on EC 3.4.22.33 - Fruit bromelain
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3.5
-
benzoyl-Ala-Leu, benzoyl-Ala-Phe, enzyme form FBA
3.5 - 4
-
benzoyl-Glu-Tyr, benzoyl-Glu-Phe
4
-
benzoyl-Ala-Leu, enzyme form FBA
2.9
optimum activity at pH 2.9 for hemoglobin
2.9
with hemoglobin as substrate
6
-
6.5
-
benzoyl-Arg amide, enzyme form FBA
6.5
optimum activity at pH 7.5 for azocasein and sodium caseinate
6.5
with azocasein and sodium caseinate as substrate
7
-
assay at
7.5
assay at
7.5
optimum activity at pH 7.5 for azoalbumin
7.5
with azoalbumin as substrate
7.7
optimum activity at pH 7.7 for casein
7.7
with casein as substrate
additional information
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additional information
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pI: 4.6