3.4.22.33: Fruit bromelain

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For detailed information about Fruit bromelain, go to the full flat file.

Reaction

Hydrolysis of proteins with broad specificity for peptide bonds. Bz-Phe-Val-Arg-/-NHMec is a good synthetic substrate, but there is no action on Z-Arg-Arg-NHMec (c.f. stem bromelain) =

Synonyms

ACMD2_17643, Ananase, Bromelain, bromelain A, bromelain B, Bromelain, juice, Bromelase, Bromelin, EC 3.4.22.4, EC 3.4.22.5, EC 3.4.4.24, Extranase, fastuosain, FBM, Fruit bromelain, Fruit bromelain FA2, Juice bromelain, More, Pinase, Traumanase

ECTree

     3 Hydrolases

         3.4 Acting on peptide bonds (peptidases)

             3.4.22 Cysteine endopeptidases

                3.4.22.33 Fruit bromelain

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Results	in table
7	Activating Compound
99	AA Sequence
8	Application
1	CAS Registry Number
1	Cloned(Commentary)
7	General Information
3	General Stability
31	Inhibitors
1	Ki Value [mM]
10	KM Value [mM]
3	Metals/Ions
19	Molecular Weight [Da]
3	Natural Substrates/ Products (Substrates)
39	Organism
26	pH Optimum
5	pH Range
1	pH Stability
5	pI Value
2	Posttranslational Modification
19	Purification (Commentary)
1	Reaction
3	Reaction Type
39	Reference
51	Source Tissue
9	Specific Activity [micromol/min/mg]
82	Substrates and Products (Substrate)
8	Subunits
27	Synonyms
19	Temperature Optimum [°C]
1	Temperature Range [°C]
10	Temperature Stability [°C]
3	Turnover Number [1/s]

Temperature Stability

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Temperature Stability on EC 3.4.22.33 - Fruit bromelain

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TEMPERATURE STABILITY

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

-4

Ananas comosus

O23791

bromelain retains 75% of its activity when stored without preservative at -4°C

731210

37

2 entries

40 - 50

2 entries

60

3 entries

70

2 entries

37

Bromelia karatas

-

partially purified enzyme, 120 min, pH 7.0, completely stable at

753642

37

Bromelia pinguin

-

partially purified enzyme, 120 min, pH 7.0, completely stable at

753642

40 - 50

Bromelia karatas

-

partially purified enzyme, 120 min, pH 7.0, loss of 20% activity

753642

40 - 50

Bromelia pinguin

-

partially purified enzyme, 120 min, pH 7.0, loss of 20% activity

753642

60

Ananas comosus

-

inactivation increases if temperature exceeds 60°C

717050

60

Bromelia karatas

-

partially purified enzyme, 120 min, pH 7.0, loss of 50% activity

753642

60

Bromelia pinguin

-

partially purified enzyme, 120 min, pH 7.0, loss of 30% activity

753642

70

Bromelia karatas

-

partially purified enzyme, 120 min, pH 7.0, loss of 70% activity

753642

70

Bromelia pinguin

-

partially purified enzyme, 120 min, pH 7.0, loss of 80% activity

753642