3.4.22.33: Fruit bromelain
This is an abbreviated version!
For detailed information about Fruit bromelain, go to the full flat file.
Reaction
Hydrolysis of proteins with broad specificity for peptide bonds. Bz-Phe-Val-Arg-/-NHMec is a good synthetic substrate, but there is no action on Z-Arg-Arg-NHMec (c.f. stem bromelain) =
Synonyms
ACMD2_17643, Ananase, Bromelain, bromelain A, bromelain B, Bromelain, juice, Bromelase, Bromelin, EC 3.4.22.4, EC 3.4.22.5, EC 3.4.4.24, Extranase, fastuosain, FBM, Fruit bromelain, Fruit bromelain FA2, Juice bromelain, More, Pinase, Traumanase
ECTree
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Substrates Products
Substrates Products on EC 3.4.22.33 - Fruit bromelain
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REACTION DIAGRAM
Benzoyl-Gly-Gly-Lys + H2O
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enzyme form FBA is much more active than FBB
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?
benzoyl-Phe-Val-Arg-4-methylcoumarin 7-amide + H2O
benzoyl-Phe-Val-Arg + 7-amino-4-methylcoumarin
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convenient substrate for fruit bromelain, scarcely affected by stem bromelain
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-
?
benzoyl-Phe-Val-Arg-p-nitroanilide + H2O
benzoyl-Phe-Val-Arg + p-nitroaniline
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-
-
-
?
benzyl-Phe-Val-Arg-4-nitroanilide + H2O
benzyl-Phe-Val-Arg + 4-nitroaniline
preferred substrate of fruit bromelain
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?
Benzyloxycarbonyl-Ala-Leu + H2O
Benzyloxycarbonyl-Ala + Leu
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enzyme form FBA is much more active than FBB
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?
benzyloxycarbonyl-Arg-Arg-4-nitroanilide + H2O
benzyloxycarbonyl-Arg-Arg + 4-nitroaniline
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?
benzyloxycarbonyl-Arg-Arg-p-nitroanilide + H2O
benzyloxycarbonyl-Arg-Arg + p-nitroaniline
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-
-
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?
Benzyloxycarbonyl-Gly-Leu + H2O
Benzyloxycarbonyl-Gly + Leu
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enzyme form FBA is much more active than FBB
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?
Benzyloxycarbonyl-Gly-Val + H2O
Benzyloxycarbonyl-Gly + Val
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enzyme form FBA is much more active than FBB
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?
bromelain + H2O
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rapid self-digestion in aqueous solution, can be prevented by addition of alpha2-macroglobulin
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?
Carbobenzoxy-Gly-Ala + H2O
Carbobenzoxy-Gly + Ala
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enzyme form FBA is much more active than FBB
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?
epsilon-NH2-Cap-Ala-Cys(S-Bzl)-7-amido-4-methylcoumarin + H2O
?
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?
epsilon-NH2-Cap-Cys(S-Bzl)-Leu-7-amido-4-methylcoumarin + H2O
?
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?
epsilon-NH2-Cap-Leu-Cys(S-Bzl)-7-amido-4-methylcoumarin + H2O
?
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?
epsilon-NH2-Cap-Pro-Cys(S-Bzl)-7-amido-4-methylcoumarin + H2O
?
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?
N-alpha-benzoyl-DL-arginine-2-naphthylamide + H2O
N-alpha-benzoyl-DL-arginine + 2-naphthylamine
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-
?
Nalpha-Benzoyl-Arg amide + H2O
Nalpha-Benzoyl-Arg + NH3
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?
Nalpha-Benzoyl-DL-Arg 4-nitroanilide + H2O
Nalpha-Benzoyl-DL-Arg + 4-nitroaniline
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?
hydrolyzed angiotensin II
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cleavage between Tyr4-Ile5
cleavage between Tyr4-Ile5
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angiotensin II + H2O
hydrolyzed angiotensin II
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cleavage between Tyr4-Ile5
cleavage between Tyr4-Ile5
?
fragments of bradykinin
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cleavage between Gly4-Phe5 and Phe5-Ser6
cleavage between Gly4-Phe5 and Phe5-Ser6
?
bradykinin + H2O
fragments of bradykinin
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cleavage between Gly4-Phe5 and Phe5-Ser6
cleavage between Gly4-Phe5 and Phe5-Ser6
?
casein + H2O
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enzyme is assayed using casein as a substrate with Coomassie dye to track completion of hydrolysis of casein, casein is hydrolysed to completion leading to a brown coloured solution, assay method development as a Bradford dye-binding-bromelain-casein system as an assay for heavy metals, based on colour development and its inhibition by Hg2+ and Cu2+
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additional information
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enzyme catalyzes synthesis of acylamino acid anilides
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additional information
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bromelain has a profound immunmodulatory effect, overview, bromelain affects blood coagulation, fibrinolysis, and platelet functions, mechanism, overview
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additional information
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enzyme is scarcely able to hydrolyze benzyloxycarbonyl-Arg-Arg-4-methylcoumarin 7-amide
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additional information
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bromelain is a cysteine protease containing a single thiol group in the active sites
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