3.4.22.33: Fruit bromelain
This is an abbreviated version!
For detailed information about Fruit bromelain, go to the full flat file.
Word Map on EC 3.4.22.33
Reaction
Hydrolysis of proteins with broad specificity for peptide bonds. Bz-Phe-Val-Arg-/-NHMec is a good synthetic substrate, but there is no action on Z-Arg-Arg-NHMec (c.f. stem bromelain) =
Synonyms
ACMD2_17643, Ananase, Bromelain, bromelain A, bromelain B, Bromelain, juice, Bromelase, Bromelin, EC 3.4.22.4, EC 3.4.22.5, EC 3.4.4.24, Extranase, fastuosain, FBM, Fruit bromelain, Fruit bromelain FA2, Juice bromelain, More, Pinase, Traumanase
ECTree
3.4.22.33 Fruit bromelainAdvanced search results
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results (Purification
Purification on EC 3.4.22.33 - Fruit bromelain
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PURIFICATION (Commentary) 
ORGANISM
UNIPROT
LITERATURE
bromelain from pineapple stem and skin are recovered by a PEG 4000/phosphate aqueous two-phase systems (ATPs) liquid-liquid extraction
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CM-Sepharose column chromatography, SP-Sepharose column chromatography, and Sephadex G-50 gel filtration
high-speed counter-current chromatography coupled with the reverse micelle solvent system consisting of 0.10 g/ml cetyltrimethylammonium bromide (CTAB)/isooctane-hexylalcohol and 0.05 M sodium phosphate buffer is successfully applied to separate bromelain from pineapple fruit
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native enzyme 7.23fold from fruits by ammonium sulfate fractionation, dialysis, and anion exchange chromatography
native enzyme from fruits by ion exchange chromatography and ultrafiltration
native enzyme partially from ripe fruits by lyophilization and gel filtration
using centrifugation, salt precipitation technique, dialysis, ion-exchange chromatography. Ion exchange chromatography using diethylaminoethyl cellulose (DEAE) anion exchangers maintain the structural integrity of purified bromelain and thereby the product exhibits better proteolytic activity than crude extract
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