3.4.22.33: Fruit bromelain

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For detailed information about Fruit bromelain, go to the full flat file.

Word Map on EC 3.4.22.33

3.4.22.33

pineapple

papain

ficin

comosus

antiglobulin

coombs

pharmacology

medicine

Reaction

Hydrolysis of proteins with broad specificity for peptide bonds. Bz-Phe-Val-Arg-/-NHMec is a good synthetic substrate, but there is no action on Z-Arg-Arg-NHMec (c.f. stem bromelain) =

Synonyms

ACMD2_17643, Ananase, Bromelain, bromelain A, bromelain B, Bromelain, juice, Bromelase, Bromelin, EC 3.4.22.4, EC 3.4.22.5, EC 3.4.4.24, Extranase, fastuosain, FBM, Fruit bromelain, Fruit bromelain FA2, Juice bromelain, More, Pinase, Traumanase

ECTree

3 Hydrolases

3.4 Acting on peptide bonds (peptidases)

3.4.22 Cysteine endopeptidases

3.4.22.33 Fruit bromelain

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Results	in table
7	Activating Compound
99	AA Sequence
8	Application
1	CAS Registry Number
1	Cloned(Commentary)
7	General Information
3	General Stability
31	Inhibitors
1	Ki Value [mM]
10	KM Value [mM]
3	Metals/Ions
19	Molecular Weight [Da]
3	Natural Substrates/ Products (Substrates)
39	Organism
26	pH Optimum
5	pH Range
1	pH Stability
5	pI Value
2	Posttranslational Modification
19	Purification (Commentary)
1	Reaction
3	Reaction Type
39	Reference
51	Source Tissue
9	Specific Activity [micromol/min/mg]
82	Substrates and Products (Substrate)
8	Subunits
27	Synonyms
19	Temperature Optimum [°C]
1	Temperature Range [°C]
10	Temperature Stability [°C]
3	Turnover Number [1/s]

Molecular Weight

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Molecular Weight on EC 3.4.22.33 - Fruit bromelain

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18000

Ananas comosus

Ananas comosus, fruit bromelain A, gel filtration

30277

19000

Ananas comosus

Ananas comosus, fruit bromelain C, gel filtration

21000 - 31000

23000

25000

27000

x * 23000, Ananas comosus, enzyme form FBA, SDS-PAGE, x * 27000, Ananas comosus, enzyme form FBB, SDS-PAGE

28000

30000

SDS-PAGE

31000

32500

Ananas comosus, gel filtration

34000

SDS-PAGE

66200 - 97000