3.4.22.30: Caricain

This is an abbreviated version!
For detailed information about Caricain, go to the full flat file.

Reaction

hydrolysis of proteins with broad specificity for peptide bonds, similar to those of papain and chymopapain =

Synonyms

caricain, Chymopapain S, More, Papaya peptidase A, Papaya peptidase II, Papaya proteinase 3, Papaya proteinase A, Papaya proteinase III, Papaya proteinase OMEGA, PPIII, PpOmega, procaricain, Proteinase omega, Proteinase, papaya A, Proteinase, papaya, III

ECTree

     3 Hydrolases

         3.4 Acting on peptide bonds (peptidases)

             3.4.22 Cysteine endopeptidases

                3.4.22.30 Caricain

Advanced search results

Do not include text mining results

Include results (more...)

Include results (more...)

Results	in table
1	Activating Compound
1	AA Sequence
3	Application
1	CAS Registry Number
2	Cloned(Commentary)
1	Crystallization (Commentary)
2	General Information
7	Inhibitors
18	KM Value [mM]
2	Localization
7	Molecular Weight [Da]
10	Natural Substrates/ Products (Substrates)
34	Organism
2	PDB ID
7	pH Optimum
1	pH Range
1	pH Stability
1	Posttranslational Modification
4	Protein Variants
9	Purification (Commentary)
7	Reaction
10	Reaction Type
33	Reference
1	Renatured (Commentary)
27	Source Tissue
1	Specific Activity [micromol/min/mg]
1	Storage Stability
41	Substrates and Products (Substrate)
4	Subunits
23	Synonyms
5	Temperature Optimum [°C]
3	Temperature Stability [°C]
8	Turnover Number [1/s]

Purification

print visible entries

print all entries

Go back to the abbreviated version
of the Enzyme Summary Page.

Purification on EC 3.4.22.30 - Caricain

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.

top print hide Go to Purification (commentary) Search

Please wait a moment until the data is sorted. This message will disappear when the data is sorted.

PURIFICATION (Commentary)

ORGANISM

UNIPROT

LITERATURE

-

Carica papaya

-

30260, 670804

CM Sephadex C-50 column chromatography

Carica papaya

P10056

731854

enzyme is semi-purified with from latex crude extracts by ion exchange chromatography on a CM Sephadex column C-50 and gel filtration

Carica papaya

P10056

695532

native enzyme from latex

Carica papaya

P10056

753097

recombinant wild-type and mutant enzymes from Escherichia coli, to homogeneity

Carica papaya

P10056

651250

to homogeneity

Carica papaya

-

649880

to homogeneity, several ligands possible for affinity chromatography are presented, fractionation on hydrophobic and cation-exchange supports, overview

Carica papaya

-

652619

with monoclonal antibodies

Carica papaya

-

30256