3.4.22.3: ficain
This is an abbreviated version!
For detailed information about ficain, go to the full flat file.
Word Map on EC 3.4.22.3
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3.4.22.3
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papain
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bromelain
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proteinases
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cathepsins
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chymotrypsin
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ficus
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cystatins
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latex
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alpha-chymotrypsin
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alloantibody
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chymopapain
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phytocystatins
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active-centre
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actinidin
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analysis
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antiglobulin
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medicine
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food industry
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synthesis
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2,2\'-dipyridyl
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industry
- 3.4.22.3
- papain
- bromelain
- proteinases
- cathepsins
- chymotrypsin
- ficus
- cystatins
- latex
- alpha-chymotrypsin
-
alloantibody
- chymopapain
-
phytocystatins
-
active-centre
- actinidin
- analysis
-
antiglobulin
- medicine
- food industry
- synthesis
-
2,2\'-dipyridyl
- industry
Reaction
Similar to that of papain =
Synonyms
debricin, EC 3.4.4.12, ficin, ficin A, ficin B, ficin C, ficin D1, ficin D2, ficin E, ficin S, higueroxyl Delabarre
ECTree
3.4.22.3 ficainAdvanced search results
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results (Purification
Purification on EC 3.4.22.3 - ficain
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PURIFICATION (Commentary) 
ORGANISM
UNIPROT
LITERATURE
a major ficin from the commercial crude proteinase mixture preparation of Ficus carica is purified by using a SP-Sepharose cation-exchanger column on fast protein liquid chromatography
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combination of cation-exchange chromatography on SP-Sepharose Fast Flow, Thiopropyl Sepharose 4B and FPLC-gel filtration chromatography
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further purification of a commercial latex preparation by cation exchange chromatography, and 2,2'-dipyridyl disulfide-glutathione or aminophenylmercuric acetate affinity chromatography, or by Gly-Phe-NH-methylcyanide affinity chromatography with elution by 2,2'-dipyridyl disulfide
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three-phase partitioning is applied for purification and recovery of ficin from Ficus carica latex. The enzyme is purified to 6.04fold with 167% recovery
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using HPLC chromatography, ultrafiltration, ion-exchange chromatography and gel filtration
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