3.4.22.3: ficain

This is an abbreviated version!
For detailed information about ficain, go to the full flat file.

Word Map on EC 3.4.22.3

3.4.22.3

papain

bromelain

proteinases

cathepsins

chymotrypsin

ficus

cystatins

latex

alpha-chymotrypsin

alloantibody

chymopapain

phytocystatins

active-centre

actinidin

analysis

antiglobulin

medicine

food industry

synthesis

2,2\'-dipyridyl

industry

Reaction

Similar to that of papain =

Synonyms

debricin, EC 3.4.4.12, ficin, ficin A, ficin B, ficin C, ficin D1, ficin D2, ficin E, ficin S, higueroxyl Delabarre

ECTree

3 Hydrolases

3.4 Acting on peptide bonds (peptidases)

3.4.22 Cysteine endopeptidases

3.4.22.3 ficain

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Results	in table
4	Activating Compound
9	AA Sequence
7	Application
1	CAS Registry Number
3	Crystallization (Commentary)
4	General Stability
46	Inhibitors
1	kcat/KM [mM/s]
1	Ki Value [mM]
37	KM Value [mM]
1	Localization
9	Metals/Ions
19	Molecular Weight [Da]
2	Natural Substrates/ Products (Substrates)
3	Organic Solvent Stability
47	Organism
8	PDB ID
13	pH Optimum
6	pH Range
2	pH Stability
5	Posttranslational Modification
20	Purification (Commentary)
2	Reaction
2	Reaction Type
47	Reference
31	Source Tissue
15	Specific Activity [micromol/min/mg]
6	Storage Stability
127	Substrates and Products (Substrate)
7	Subunits
29	Synonyms
8	Temperature Optimum [°C]
2	Temperature Range [°C]
22	Temperature Stability [°C]
32	Turnover Number [1/s]

Organic Solvent Stability

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Organic Solvent Stability on EC 3.4.22.3 - ficain

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Ethanol

Ficus carica

12% (v/v), 4°C, pH 3.2, 530 h, about 35% loss of activity

752461

guanidine-HCl

Ficus carica

ficin loses its complete structure in presence of 4 M guanidine-HCl at pH 7.0, ficin loses 50% activity at around 1 M guanidine-HCl, and is complete inactivated at 3 M

718344

urea

Ficus carica

the enzyme activity decreases significantly at low concentration of urea before unfolding of the enzyme molecule (about 50% residual activity at 2 M, about 20% residual activity at 4 M, no activity at 6 M)

718344