3.4.22.25: glycyl endopeptidase

This is an abbreviated version!
For detailed information about glycyl endopeptidase, go to the full flat file.

Reaction

preferential cleavage: Gly-/-, in proteins and small molecule substrates =

Synonyms

Chymopapain, Chymopapain M, CpXCP7, glycine endopeptidase, Glycyl endopeptidase, More, Papaya peptidase B, Papaya proteinase 4, Papaya proteinase IV, PPIV, Proteinase, glycine-specific

ECTree

     3 Hydrolases

         3.4 Acting on peptide bonds (peptidases)

             3.4.22 Cysteine endopeptidases

                3.4.22.25 glycyl endopeptidase

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Results	in table
1	Activating Compound
4	AA Sequence
2	Application
2	CAS Registry Number
3	Cloned(Commentary)
1	Crystallization (Commentary)
2	General Information
21	Inhibitors
2	Ki Value [mM]
3	KM Value [mM]
4	Localization
6	Molecular Weight [Da]
4	Natural Substrates/ Products (Substrates)
21	Organism
1	PDB ID
6	pH Optimum
1	pH Range
1	pH Stability
4	Posttranslational Modification
2	Protein Variants
9	Purification (Commentary)
2	Reaction
3	Reaction Type
21	Reference
13	Source Tissue
35	Substrates and Products (Substrate)
2	Subunits
21	Synonyms
1	Temperature Optimum [°C]
2	Temperature Stability [°C]
3	Turnover Number [1/s]

Substrates Products

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Substrates Products on EC 3.4.22.25 - glycyl endopeptidase

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SUBSTRATE

PRODUCT                       

REACTION DIAGRAM

ORGANISM

UNIPROT

COMMENTARY
(Substrate)

LITERATURE
(Substrate)

COMMENTARY
(Product)

LITERATURE
(Product)

Reversibility
r=reversible
ir=irreversible
?=not specified

aggrecan + H2O

?

Carica papaya

-

-

30148

-

-

?

azocasein + H2O

trichloroacetic acid-soluble peptides from azocasein

Carica papaya

-

10% of the activity of papain

30149

-

30149

?

benzyloxycarbonyl-Gly-Leu-NH + H2O

L-leucine amide + N-benzyloxycarbonyl-glycine

Carica papaya

P05994

-

679571

-

-

?

benzyloxycarbonyl-Gly-Phe-NH + H2O

L-phenylalanine amide + N-benzyloxycarbonyl-glycine

Carica papaya

P05994

-

679571

-

-

?

benzyloxycarbonyl-Gly-Tyr-NH + H2O

L-tyrosine amide + N-benzyloxycarbonyl-glycine

Carica papaya

P05994

-

679571

-

-

?

benzyloxycarbonyl-Gly-Tyr-Oet + H2O

L-tyrosine ethyl ester + N-benzyloxycarbonyl-glycine

Carica papaya

P05994

-

679571

-

-

?

casein + H2O

?

Carica papaya

-

-

733902

-

-

?

casein + H2O

trichloroacetic acid-soluble peptides derived from casein

Carica papaya

-

10% of the activity of papain

30149

-

30149

?

Chicken cystatin + H2O

?

Carica papaya

-

-

30145, 668767

-

-

?

Gly-4-nitroanilide + H2O

glycine + 4-nitroaniline

Carica papaya

-

-

668767

-

-

?

Hemoglobin + H2O

Trichloroacetic acid-soluble peptides from haemoglobin

Carica papaya

-

-

30149

-

30149

?

hide powder azure + H2O

?

Carica papaya

-

-

30149, 30154

-

-

?

Human cystatin C + H2O

?

Carica papaya

-

-

30145, 668767

-

-

?

N-Acetyl-L-Phe-Gly 4-nitroanilide + H2O

?

Carica papaya

-

-

30153

-

-

?

N-Benzyloxycarbonyl-Gly 4-nitrophenyl ester + H2O

?

Carica papaya

-

-

30142

-

-

?

protein + H2O

peptides

2 entries

tert-Butyloxycarbonyl-Ala-Ala-Ala 4-methylcoumarin 7-amide + H2O

?

Carica papaya

-

-

30144

-

-

?

tert-Butyloxycarbonyl-Ala-Ala-Gly 4-methylcoumarin 7-amide + H2O

?

Carica papaya

-

-

30144, 30146

-

-

?

tert-Butyloxycarbonyl-Ala-Ala-Gly 4-nitroanilide + H2O

?

Carica papaya

-

-

30144, 30146, 30150

-

-

?

tert-butyloxycarbonyl-Ala-Ala-Gly-p-nitroanilide + H2O

tert-butyloxycarbonyl-Ala-Ala-Gly + p-nitroaniline

Carica papaya

P05994

-

679571

-

-

?

Trypsin + H2O

Hydrolyzed trypsin

Carica papaya

-

16 cleavage points of which 13 are glycyl bonds

30144

-

30144

?

additional information

?

-

7 entries

protein + H2O

peptides

Carica papaya

-

-

651248

-

651248

?

protein + H2O

peptides

Carica papaya

-

-

652619

-

652619

?

additional information

?

-

Carica papaya

-

synthetic substrates of other papaya proteinases

30149

-

-

?

additional information

?

-

Carica papaya

-

absolute specificity for substrates with glycine at the P1 position (structural origin of the unusual specificity)

30151

-

-

?

additional information

?

-

Carica papaya

-

not: cystatin A

30145

-

-

?

additional information

?

-

Carica papaya

-

the recombinant proregion of the enzyme can inhibit the cysteine protease of larvae of the Colorado beetle, Leptinotarsa decemlineata, analoguesly to specific inhibitor oryzacystatin I, inhibitory effect is affected by temperature

651248

-

651248

?

additional information

?

-

Carica papaya

-

the enzyme is useful for the plant defense system

668767

-

-

?

additional information

?

-

Carica papaya

-

substrate specificity with preference for glycyl residues, structure-function relationship, overview, hydrolyzes glycine esters but not Bz-Arg-4-nitroanilide

668767

-

-

?

additional information

?

-

Carica papaya

P05994

glycyl endopeptidase fails to hydrolyse DL-benzyl-Arg-p-nitroanilide

679571

-

-

?