3.4.22.25: glycyl endopeptidase

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For detailed information about glycyl endopeptidase, go to the full flat file.

Reaction

preferential cleavage: Gly-/-, in proteins and small molecule substrates =

Synonyms

Chymopapain, Chymopapain M, CpXCP7, glycine endopeptidase, Glycyl endopeptidase, More, Papaya peptidase B, Papaya proteinase 4, Papaya proteinase IV, PPIV, Proteinase, glycine-specific

ECTree

     3 Hydrolases

         3.4 Acting on peptide bonds (peptidases)

             3.4.22 Cysteine endopeptidases

                3.4.22.25 glycyl endopeptidase

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Results	in table
1	Activating Compound
4	AA Sequence
2	Application
2	CAS Registry Number
3	Cloned(Commentary)
1	Crystallization (Commentary)
2	General Information
21	Inhibitors
2	Ki Value [mM]
3	KM Value [mM]
4	Localization
6	Molecular Weight [Da]
4	Natural Substrates/ Products (Substrates)
21	Organism
1	PDB ID
6	pH Optimum
1	pH Range
1	pH Stability
4	Posttranslational Modification
2	Protein Variants
9	Purification (Commentary)
2	Reaction
3	Reaction Type
21	Reference
13	Source Tissue
35	Substrates and Products (Substrate)
2	Subunits
21	Synonyms
1	Temperature Optimum [°C]
2	Temperature Stability [°C]
3	Turnover Number [1/s]

Purification

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Purification on EC 3.4.22.25 - glycyl endopeptidase

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PURIFICATION (Commentary)

ORGANISM

UNIPROT

LITERATURE

-

Carica papaya

-

30145, 30148, 30149

ammonium sulfate precipitation

Carica papaya

P05994

679571

different methods, overview

Carica papaya

-

668767

glycyl endopeptidase from papaya latex is partitioned using aqueous two-phase (10% PEG 6000- 10% (NH4)2SO4) in combination with NH4(SO4)2 precipitation.The partially purified glycyl endopeptidase shows the potential in production of antioxidative gelatin hydrolysates. The enzyme fraction contain lower odorous compounds in papaya latex. The gelatin hydrolysate produced using the selected fraction have negligible odorous compounds

Carica papaya

-

733902

native enzyme from latex

Carica papaya

P05994

753097

native enzyme from latex by ion exchange and adsorption chromatography, and gel filtration

Carica papaya

-

667431

several ligands possible for affinity chromatography are presented, fractionation on hydrophobic and cation-exchange supports, overview

Carica papaya

-

652619