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3.4.22.1: cathepsin B

This is an abbreviated version!
For detailed information about cathepsin B, go to the full flat file.

Word Map on EC 3.4.22.1

Reaction

Hydrolysis of proteins with broad specificity for peptide bonds. Preferentially cleaves -Arg-Arg-/- bonds in small molecule substrates (thus differing from cathepsin L). In addition to being an endopeptidase, shows peptidyl-dipeptidase activity, liberating C-terminal dipeptides =

Synonyms

AC-5, AC-cathB-1, AC-cathB-2, APP secretase, cat B, CatB, CatB1, Cath B, Cath-B, CathB, cathepsin B, cathepsin B proteinase, cathepsin B-like AC-5, cathepsin B-like counter-defence protein, cathepsin B-like cysteine protease, cathepsin B-like cysteine protease 1, cathepsin B-type cysteine protease, cathepsin B1, cathepsin B2, cathepsin B5, cathepsin Ba, cathepsin II, cathepsin-B, CmCatB1, CmCatB2, CP-2, CPB, CpCathB, CPR-4, CsCB1, CsCB2, CsCB3, CsCB4, CTB, CTSB, cysteine cathepsin, DEVDase, LycCatB, MmeCB, More, Na-CP-2, Na-CP-3, Na-CP-4, Na-CP-5, NbCathB, nfcpb, nfcpb-L, PcCTSB, rFgCatB2, Sm31, SmCatB, SmCB1, toxopain-1, TrCB1.1, TrCB1.2, TrCB1.3, TrCB1.4, TrCB1.5, TrCB1.6, TsCPB2

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.22 Cysteine endopeptidases
                3.4.22.1 cathepsin B

Crystallization

Crystallization on EC 3.4.22.1 - cathepsin B

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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
hanging drop vapour diffusion method, in complex with inhibitors, in 50 mM citrate buffer (pH 3.5) containing 2.2-2.6 M sodium phosphate
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in complex with inhibitor CA074
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in complex with inhibitor E64c
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purified wild-type enzyme and enzyme mutants in complex with inhibitor chagasin, hanging drop vapor diffusion method at 18°C, for crystal form I: mixing of 0.001 ml of 5 mg/ml chagasin solution with 0.002 ml of protein solution containing 3 mg/ml cathepsin B, and 0.002 ml of precipitant solution containing 0.2 M NH4H2PO4, 20% PEG 3350, pH 4.6, 2 weeks, for crystal form II: mixing of 0.001 mL of 5 mg/ml chagasin solution with 0.0015 ml of protein solution containing 3 mg/ml cathepsin B, and 0.001 ml of precipitant solution containing 0.14 M NH4F, 14% PEG 3350, pH 6.2, 1 week, X-ray diffraction structure determination and analysis at 1.8-2.7 A resolution
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