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3.4.22.1: cathepsin B

This is an abbreviated version!
For detailed information about cathepsin B, go to the full flat file.

Word Map on EC 3.4.22.1

Reaction

Hydrolysis of proteins with broad specificity for peptide bonds. Preferentially cleaves -Arg-Arg-/- bonds in small molecule substrates (thus differing from cathepsin L). In addition to being an endopeptidase, shows peptidyl-dipeptidase activity, liberating C-terminal dipeptides =

Synonyms

AC-5, AC-cathB-1, AC-cathB-2, APP secretase, cat B, CatB, CatB1, Cath B, Cath-B, CathB, cathepsin B, cathepsin B proteinase, cathepsin B-like AC-5, cathepsin B-like counter-defence protein, cathepsin B-like cysteine protease, cathepsin B-like cysteine protease 1, cathepsin B-type cysteine protease, cathepsin B1, cathepsin B2, cathepsin B5, cathepsin Ba, cathepsin II, cathepsin-B, CmCatB1, CmCatB2, CP-2, CPB, CpCathB, CPR-4, CsCB1, CsCB2, CsCB3, CsCB4, CTB, CTSB, cysteine cathepsin, DEVDase, LycCatB, MmeCB, More, Na-CP-2, Na-CP-3, Na-CP-4, Na-CP-5, NbCathB, nfcpb, nfcpb-L, PcCTSB, rFgCatB2, Sm31, SmCatB, SmCB1, toxopain-1, TrCB1.1, TrCB1.2, TrCB1.3, TrCB1.4, TrCB1.5, TrCB1.6, TsCPB2

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.22 Cysteine endopeptidases
                3.4.22.1 cathepsin B

Cloned

Cloned on EC 3.4.22.1 - cathepsin B

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
baculovirus expression system
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CatB DNA and amino acid sequence determination and analysis, recombinant expression in Spodoptera frugiperda cell line Sf9 using the baculovirus expression vector system with the Autographa californica nucleopolyhedrovirus
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cloning from a hepatopancreas cDNA library of wild-type strain, adult and Schistosoma mansoni-resistant, BS-90 snails, DNA and amino acid sequence determination and analysis, quantitative expression analysis by real-time RT-PCR
expressed as a His-tagged fusion protein in Escherichia coli
expressed as a His-tagged fusion protein in Escherichia coli. Recombinant CsCBs induces high IgG titers in rats, indicating high immunogenicity of CsCB family
expressed in Escherichia coli
expressed in Escherichia coli BL21 cells
expressed in Escherichia coli BL21(DE3) cells
expressed in murine melanoma cell line B16-F10
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expression analysis by quantitative RT-PCR, expression patterns of cathepsin B and endogenous inhibitor cystatin C
expression analysis by real-time RT-PCR
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expression analysis by realtime PCR, regulation of enzyme expression takes place at the transcriptional level, transcriptional transactivation of the NF-kappaB-responsive promoter which is higher in cells with mitochondria depleted in mtDNA
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expression analysis by semi-quantitative RT-PCR in healthy and fulminant hepatic failure livers
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expression in CABA I ovarian carcinoma cells, cathepsin B expression analysis by real-time PCR
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expression in Escherichia coli
expression in Escherichia coli BL21(DE3)
expression in Escherichia coli, expressed as zymogen in Pichia pastoris
expression in inclusion bodies in Escherichia coli
expression in inclusion bodies in Escherichia coli, cathepsin B gene is located on chromosome 14 of the mouse genome
expression in Pichia pastoris
expression in Saccharomyces cerevisiae
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expression of His-tagged pro-catB1 in Pichia pastoris
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expression of mutant enzymes in Pichia pastoris
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expression of mutants as inactive pro-proteins in Pichia pastoris
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expression of recombinant human procathepsin B and cathepsin B in Escherichia coli
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functional expression of His-tagged wild-type CatB in Pichia pastoris, and expression of some His-tagged glycosylation site mutants. Residue Asn236 appears necessary for protein expression
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gene CmCatB1, DNA and amino acid sequence determination and analysis, the native signal peptide of CmCatB1 is replaced by a yeast alpha-factor secretion signal sequence, the yeast peptide is subsequently cleaved and the recombinant C-terminally His6-tagged CmCatB1 proprotein is secreted to the cell culture medium of the transformed Pichia pastoris cells
gene CmCatB2, DNA and amino acid sequence determination and analysis, CmCatB2 may be an inactive protease isoform resulting from splicing errors
gene PoCatB, cDNA is isolated from flounder embryonic cells treated with UV-inactivated grass carp hemorrhage virus, DNA and amino acid sequence determination and analysis
heterologous expression of NbCathB in insect cells
intestine-specific expression of GFP-tagged mature cathepsin B driven by the A33-antigen promoter, that contains positive cis-regulatory elements, including caudal-related homeobox binding sites, in CHO-K1 cells, evaluation of the expression system, overview
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procathepsin B
ProFgCB5 is expressed as a recombinant, N-terminal His-tagged protein in Escherichia coli M15. Expression in Pichia pastoris
quantitation of cysteine protease gene expression in different developmental stages, sequence comparison and phylogenetic analysis, overview. Expression of Na-CP-2 in insoluble inclusion bodies in Escherichia coli
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quantitation of cysteine protease gene expression in different developmental stages, sequence comparison and phylogenetic analysis, overview. Expression of Na-CP-3 in soluble, secreted form in Pichia pastoris strain X-33
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quantitation of cysteine protease gene expression in different developmental stages, sequence comparison and phylogenetic analysis, overview. Expression of Na-CP-4 in insoluble inclusion bodies in Escherichia coli
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quantitation of cysteine protease gene expression in different developmental stages, sequence comparison and phylogenetic analysis, overview. Expression of Na-CP-5 in insoluble inclusion bodies in Escherichia coli
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quantitative expression analysis of CATB
recombinantly expressed in Pichia pastoris
recombinantly expressed in Pichia pastoris and Escherichia coli
SmCB1 expression analysis, expression in Pichia pastoris
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the recombinant CpCathB is expressed in the Escherichia coli Rosetta-gami (DE3) strain