3.4.21.B7: mannan-binding lectin-associated serine protease 1

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For detailed information about mannan-binding lectin-associated serine protease 1, go to the full flat file.

Reaction

endopeptidase activity. It triggers the activation of complement cascade by activating the C4 and C2 components. It activates the C4 component by cleaving the alpha-chain of C4 =

Synonyms

mannan-binding lectin-associated serine protease, mannan-binding lectin-associated serine protease-1, mannose-binding lectin-associated serine protease, MASP-1, MASP1, MBL-associated serine protease, MBL-associated serine protease 1, MBL-associated serine protease-1, MBL-MASP, P100, Ra-reactive factor, RaRF, S01.198

ECTree

     3 Hydrolases

         3.4 Acting on peptide bonds (peptidases)

             3.4.21 Serine endopeptidases

                3.4.21.B7 mannan-binding lectin-associated serine protease 1

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Results	in table
4	Activating Compound
3	AA Sequence
4	Application
1	CAS Registry Number
19	Cloned(Commentary)
6	Crystallization (Commentary)
199	Disease/ Diagnostics
3	Expression
31	General Information
1	General Stability
32	Inhibitors
8	kcat/KM [mM/s]
12	Ki Value [mM]
9	KM Value [mM]
3	Localization
6	Metals/Ions
27	Molecular Weight [Da]
65	Natural Substrates/ Products (Substrates)
59	Organism
3	pH Optimum
1	pH Range
1	pI Value
5	Posttranslational Modification
9	Protein Variants
16	Purification (Commentary)
1	Reaction
24	Reaction Type
59	Reference
1	Renatured (Commentary)
35	Source Tissue
129	Substrates and Products (Substrate)
15	Subunits
69	Synonyms
3	Temperature Optimum [°C]
2	Temperature Stability [°C]
6	Turnover Number [1/s]

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RENATURED/Commentary

ORGANISM

UNIPROT

LITERATURE

solubilization of recombinant chitin-binding domain-tagged MAPS-1 CUB1-EGF-CUB2 from Escherichia coli strain BL21 (DE3) pLysS inclusion bodies by 7 M guanidinium hydrochloride in 50 mM Tris, 50 mM DTT, pH 8.0, followed by dilution to 5.5 mg/ml protein, and refolding by diluting 20 ml of the 5 mg/mL solution into 1 l of 0.75 M Arg, 200 mM CaCl2, 3 mM glutathione, 2 mM oxidized glutathione, pH 8.0, refolding buffer, at least one week at 4°C, followed by dialysis

Homo sapiens

P48740

732518