3.4.21.B7: mannan-binding lectin-associated serine protease 1

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For detailed information about mannan-binding lectin-associated serine protease 1, go to the full flat file.

Reaction

endopeptidase activity. It triggers the activation of complement cascade by activating the C4 and C2 components. It activates the C4 component by cleaving the alpha-chain of C4 =

Synonyms

mannan-binding lectin-associated serine protease, mannan-binding lectin-associated serine protease-1, mannose-binding lectin-associated serine protease, MASP-1, MASP1, MBL-associated serine protease, MBL-associated serine protease 1, MBL-associated serine protease-1, MBL-MASP, P100, Ra-reactive factor, RaRF, S01.198

ECTree

     3 Hydrolases

         3.4 Acting on peptide bonds (peptidases)

             3.4.21 Serine endopeptidases

                3.4.21.B7 mannan-binding lectin-associated serine protease 1

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Results	in table
4	Activating Compound
3	AA Sequence
4	Application
1	CAS Registry Number
19	Cloned(Commentary)
6	Crystallization (Commentary)
199	Disease/ Diagnostics
3	Expression
31	General Information
1	General Stability
32	Inhibitors
8	kcat/KM [mM/s]
12	Ki Value [mM]
9	KM Value [mM]
3	Localization
6	Metals/Ions
27	Molecular Weight [Da]
65	Natural Substrates/ Products (Substrates)
59	Organism
3	pH Optimum
1	pH Range
1	pI Value
5	Posttranslational Modification
9	Protein Variants
16	Purification (Commentary)
1	Reaction
24	Reaction Type
59	Reference
1	Renatured (Commentary)
35	Source Tissue
129	Substrates and Products (Substrate)
15	Subunits
69	Synonyms
3	Temperature Optimum [°C]
2	Temperature Stability [°C]
6	Turnover Number [1/s]

Engineering

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Engineering on EC 3.4.21.B7 - mannan-binding lectin-associated serine protease 1

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PROTEIN VARIANTS

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

R448Q

Homo sapiens

P48740

site-directed mutagenesis

732519

R504Q

Homo sapiens

P48740

site-directed mutagenesis, construction of a MASP-1 catalytic fragment, that has a molecular weight of 45.5 kDa and consists of the three C-terminal domains complement control protein 1 and 2 followed by the serine protease domain

732693

S627A

Homo sapiens

-

kinetic parameters similiar to wild type, mutant is activated by its active counterpart

669707

S646A

2 entries

additional information

6 entries

S646A

Homo sapiens

P48740

site-directed mutagenesis

732519

S646A

Homo sapiens

P48740

site-directed mutagenesis, the mutant remains as inactive zymogen

732869

additional information

Homo sapiens

P48740

generation of a MASP-1 catalytic fragment encoding the CCP1-CCP2-SP region (rMASPcf)

732519

additional information

Homo sapiens

P48740

generation of a truncated enzyme version comprising the MAPS-1 CUB1-EGF-CUB2 domains, i.e. MASP-1 D1-3 CBD, rapid dissociation upon EDTA treatment, and heterodimer formation, exchange of subunits between dimers in the presence of Ca2+, overview. Heterodimer formation between N-terminal fragemnts of MASP1 and MASP-2, i.e. MASP-1 D1-3 and MASP-2 D1-3, is structurally allowed but takes place to only a small amount

732518

additional information

Homo sapiens

-

generation of a truncated enzyme version comprising the MAPS-1 CUB1-EGF-CUB2 domains, i.e. MASP-1 D1-3 CBD, rapid dissociation upon EDTA treatment, and heterodimer formation, exchange of subunits between dimers in the presence of Ca2+, overview. Heterodimer formation between N-terminal fragemnts of MASP1 and MASP-2, i.e. MASP-1 D1-3 and MASP-2 D1-3, is structurally allowed but takes place to only a small amount

732518

additional information

Mus musculus

P98064

generation of a MASP-1-deficient mouse model

731099

additional information

Mus musculus

-

generation of a MASP-1-deficient mouse model

731099

additional information

Rattus norvegicus

-

MASP-1 ent, catalytically active form of MASP-1 produced by substituting residues 425Lys-His-Ile-Ser-Arg

659350