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3.4.21.B6: prostasin

This is an abbreviated version!
For detailed information about prostasin, go to the full flat file.

Word Map on EC 3.4.21.B6

Reaction

endoprotease activity. Trypsin-like enzymatic activity =

Synonyms

CAP-1, CAP1, CAP1/PRSS8, channel activating protease 1, channel activating protease-1, channel-activating protease 1, channel-activating protease-1, LD47230p, prostasin, prostasin/PRSS8, PRSS8, S01.159, tracheal-prostasin

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.21 Serine endopeptidases
                3.4.21.B6 prostasin

Cloned

Cloned on EC 3.4.21.B6 - prostasin

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in FT-293 cells
-
expressed in HEK-293 cells
expressed in HEK-293T cells
-
expressed in M-1 cells
expressed in MDCK cells
-
expressed in Mus musculus urinary bladder epithelium
expressed in silkworm larvae by recombinant baculovirus
-
expressed in the prostate carcinoma cell line PC-3
-
expression in Sf9 cells
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expression of the prostasin proenzyme in Escherichia coli as insoluble inclusion bodies, refolding and activating via proteolytic removal of the N-terminal propeptide
into pCRT7/NT-TOPO and expressed in Escherichia coli BL21(DE3)pLysS, into pcDNA3.1/V5-His-TOPO and expressed in CHO cells and HEK-293 cells, overexpression in CF epithelial cells
-
into vector pREP8, expression in the bladder of transgenic mice
subcloned into pGEM-T easy vector, subcloned into pGL3-basic vector and expressed in M-1 cells
-
subcloned into the pREP8 plasmid, transfected into the HEK-293 cells
-