Any feedback?
Please rate this page
(all_enzymes.php)
(0/150)

BRENDA support

3.4.21.B57: pernisine

This is an abbreviated version!
For detailed information about pernisine, go to the full flat file.

Word Map on EC 3.4.21.B57

Reaction

the enzyme can digest the pathological prion protein isoform (PrPSc) from different species, e.g. human, bovine, deer and mouse =

Synonyms

pernisine, subtilase, Tk-SP, Tk-subtilisin, TKS

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.21 Serine endopeptidases
                3.4.21.B57 pernisine

Reference

topprint

Reference on EC 3.4.21.B57 - pernisine

Please use the Reference Search for a specific query.
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Tanaka, S.; Saito, K.; Chon, H.; Matsumura, H.; Koga, Y.; Takano, K.; Kanaya, S.
Crystallization and preliminary X-ray diffraction study of an active-site mutant of pro-Tk-subtilisin from a hyperthermophilic archaeon
Acta Crystallogr. Sect. F
62
902-905
2006
Thermococcus kodakarensis (P58502)
Manually annotated by BRENDA team
Pulido, M.A.; Tanaka, S.; Sringiew, C.; You, D.J.; Matsumura, H.; Koga, Y.; Takano, K.; Kanaya, S.
Requirement of left-handed glycine residue for high stability of the Tk-subtilisin propeptide as revealed by mutational and crystallographic analyses
J. Mol. Biol.
374
1359-1373
2007
Thermococcus kodakarensis (P58502)
Manually annotated by BRENDA team
Takeuchi, Y.; Tanaka, S.; Matsumura, H.; Koga, Y.; Takano, K.; Kanaya, S.
Requirement of a unique Ca(2+)-binding loop for folding of Tk-subtilisin from a hyperthermophilic archaeon
Biochemistry
48
10637-10643
2009
Thermococcus kodakarensis (P58502)
Manually annotated by BRENDA team
Kannan, Y.; Koga, Y.; Inoue, Y.; Haruki, M.; Takagi, M.; Imanaka, T.; Morikawa, M.; Kanaya, S.
Active subtilisin-like protease from a hyperthermophilic archaeon in a form with a putative prosequence
Appl. Environ. Microbiol.
67
2445-2452
2001
Thermococcus kodakarensis (P58502)
Manually annotated by BRENDA team
Pulido, M.; Saito, K.; Tanaka, S.; Koga, Y.; Morikawa, M.; Takano, K.; Kanaya, S.
Ca2+-dependent maturation of subtilisin from a hyperthermophilic archaeon, Thermococcus kodakaraensis: the propeptide is a potent inhibitor of the mature domain but is not required for its folding
Appl. Environ. Microbiol.
72
4154-4162
2006
Thermococcus kodakarensis (P58502)
Manually annotated by BRENDA team
Koga, Y.; Tanaka, S.; Sakudo, A.; Tobiume, M.; Aranishi, M.; Hirata, A.; Takano, K.; Ikuta, K.; Kanaya, S.
Proteolysis of abnormal prion protein with a thermostable protease from Thermococcus kodakarensis KOD1
Appl. Microbiol. Biotechnol.
98
2113-2120
2013
Thermococcus kodakarensis (P58502)
Manually annotated by BRENDA team
Uehara, R.; Takeuchi, Y.; Tanaka, S.; Takano, K.; Koga, Y.; Kanaya, S.
Requirement of Ca(2+) ions for the hyperthermostability of Tk-subtilisin from Thermococcus kodakarensis
Biochemistry
51
5369-5378
2012
Thermococcus kodakarensis (P58502)
Manually annotated by BRENDA team
Tanaka, S.; Koga, Y.; Takano, K.; Kanaya, S.
Inhibition of chymotrypsin- and subtilisin-like serine proteases with Tk-serpin from hyperthermophilic archaeon Thermococcus kodakaraensis
Biochim. Biophys. Acta
1814
299-307
2010
Thermococcus kodakarensis (P58502)
Manually annotated by BRENDA team
Hirata, A.; Hori, Y.; Koga, Y.; Okada, J.; Sakudo, A.; Ikuta, K.; Kanaya, S.; Takano, K.
Enzymatic activity of a subtilisin homolog, Tk-SP, from Thermococcus kodakarensis in detergents and its ability to degrade the abnormal prion protein
BMC Biotechnol.
13
19
2013
Thermococcus kodakarensis (P58502), Thermococcus kodakarensis
Manually annotated by BRENDA team
Catara, G.; Ruggiero, G.; La Cara, F.; Digilio, F.A.; Capasso, A.; Rossi, M.
A novel extracellular subtilisin-like protease from the hyperthermophile Aeropyrum pernix K1: biochemical properties, cloning, and expression
Extremophiles
7
391-399
2003
Aeropyrum pernix, Aeropyrum pernix DSM 11879
Manually annotated by BRENDA team
Sinsereekul, N.; Foophow, T.; Yamanouchi, M.; Koga, Y.; Takano, K.; Kanaya, S.
An alternative mature form of subtilisin homologue, Tk-SP, from Thermococcus kodakaraensis identified in the presence of Ca2+
FEBS J.
278
1901-1911
2011
Thermococcus kodakarensis (P58502), Thermococcus kodakarensis
Manually annotated by BRENDA team
Uehara, R.; Ueda, Y.; You, D.J.; Koga, Y.; Kanaya, S.
Accelerated maturation of Tk-subtilisin by a Leu->Pro mutation at the C-terminus of the propeptide, which reduces the binding of the propeptide to Tk-subtilisin
FEBS J.
280
994-1006
2013
Thermococcus kodakarensis (P58502)
Manually annotated by BRENDA team
Tanaka, S.; Takeuchi, Y.; Matsumura, H.; Koga, Y.; Takano, K.; Kanaya, S.
Crystal structure of Tk-subtilisin folded without propeptide: requirement of propeptide for acceleration of folding
FEBS Lett.
582
3875-3878
2008
Thermococcus kodakarensis (P58502)
Manually annotated by BRENDA team
Foophow, T.; Tanaka, S.; Angkawidjaja, C.; Koga, Y.; Takano, K.; Kanaya, S.
Crystal structure of a subtilisin homologue, Tk-SP, from Thermococcus kodakaraensis: requirement of a C-terminal beta-jelly roll domain for hyperstability
J. Mol. Biol.
400
865-877
2010
Thermococcus kodakarensis (P58502), Thermococcus kodakarensis
Manually annotated by BRENDA team
Snajder, M.; Vilfan, T.; Cernilec, M.; Rupreht, R.; Popovic, M.; Juntes, P.; Serbec, V.C., Ulrih, N.P.
Enzymatic degradation of PrPSc by a protease secreted from Aeropyrum pernix K1
PLoS One
7
e39548
2012
Aeropyrum pernix, Aeropyrum pernix DSM 11879
Manually annotated by BRENDA team
Yuzaki, K.; Sanda, Y.; You, D.J.; Uehara, R.; Koga, Y.; Kanaya, S.
Increase in activation rate of Pro-Tk-subtilisin by a single nonpolar-to-polar amino acid substitution at the hydrophobic core of the propeptide domain
Protein Sci.
22
1711-1721
2013
Thermococcus kodakarensis (P58502)
Manually annotated by BRENDA team
Okada, J.; Koga, Y.; Takano, K.; Kanaya, S.
Slow unfolding pathway of hyperthermophilic Tk-RNase H2 examined by pulse proteolysis using the stable protease Tk-subtilisin
Biochemistry
51
9178-9191
2012
Thermococcus kodakarensis (P58502)
Manually annotated by BRENDA team
Uehara, R.; Angkawidjaja, C.; Koga, Y.; Kanaya, S.
Formation of the high-affinity calcium binding site in pro-subtilisin E with the insertion sequence IS1 of pro-Tk-subtilisin
Biochemistry
52
9080-9088
2013
Thermococcus kodakarensis (P58502)
Manually annotated by BRENDA team
Uehara, R.; Tanaka, S.; Takano, K.; Koga, Y.; Kanaya, S.
Requirement of insertion sequence IS1 for thermal adaptation of Pro-Tk-subtilisin from hyperthermophilic archaeon
Extremophiles
16
841-851
2012
Thermococcus kodakarensis (P58502)
Manually annotated by BRENDA team
Snajder, M.; Mihelic, M.; Turk, D.; Ulrih, N.P.
Codon optimisation is key for pernisine expression in Escherichia coli
PLoS ONE
10
e0123288
2015
Aeropyrum pernix (Q9YFI3)
Manually annotated by BRENDA team