3.4.21.B57: pernisine
This is an abbreviated version!
For detailed information about pernisine, go to the full flat file.
Word Map on EC 3.4.21.B57
-
3.4.21.B57
-
hyperthermophilic
-
archaeon
-
subtilisin-like
-
pernix
-
aeropyrum
-
subtilisins
-
thermococcus
-
prion
-
medicine
-
kodakaraensis
-
proregion
-
mesophilic
-
autoprocessed
-
detergents
-
edta
-
codon-optimised
-
far-uv
-
tk-subtilisin
-
high-temperature
-
cacl2
-
n-propeptide
-
roll
-
hyperthermostable
- 3.4.21.B57
-
hyperthermophilic
- archaeon
-
subtilisin-like
- pernix
-
aeropyrum
- subtilisins
-
thermococcus
- prion
- medicine
- kodakaraensis
-
proregion
-
mesophilic
-
autoprocessed
- detergents
- edta
-
codon-optimised
-
far-uv
- tk-subtilisin
-
high-temperature
- cacl2
- n-propeptide
-
roll
-
hyperthermostable
Reaction
the enzyme can digest the pathological prion protein isoform (PrPSc) from different species, e.g. human, bovine, deer and mouse =
Synonyms
pernisine, subtilase, Tk-SP, Tk-subtilisin, TKS
ECTree
3.4.21.B57 pernisineAdvanced search results
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results (Purification
Purification on EC 3.4.21.B57 - pernisine
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PURIFICATION (Commentary) 
ORGANISM
UNIPROT
LITERATURE
mutant proteins, Deltaloop-Tk-subtilisin (Ca2+-binding loop is removed), DeltaCa2-Pro-S324A (Ca2+-binding site Ca2 is removed), and DeltaCa3-Pro-S324A (Ca2+-binding site Ca3 is removed), are purified in a Ca2+-bound form
recombinant His-tagged codon-optimised wild-type and codon-optimised S355A active site mutant from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration
