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3.4.21.B57: pernisine

This is an abbreviated version!
For detailed information about pernisine, go to the full flat file.

Word Map on EC 3.4.21.B57

Reaction

the enzyme can digest the pathological prion protein isoform (PrPSc) from different species, e.g. human, bovine, deer and mouse =

Synonyms

pernisine, subtilase, Tk-SP, Tk-subtilisin, TKS

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.21 Serine endopeptidases
                3.4.21.B57 pernisine

Molecular Weight

Molecular Weight on EC 3.4.21.B57 - pernisine

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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
23000
-
x * 23000, SDS-PAGE, the purified pernisine is in active mature form at 23 kDa and in pre-form at 34 kDa, it appears that the purified pernisin is a mixture of two different active forms
34000
35160
-
gel filtration
36000
x * 36000, activated mature codon-optimised wild-type enzyme, SDS-PAGE, x * 55000, recombinant His-tagged proform codon-optimised wild-type enzyme, SDS-PAGE
40000
gel filtration
42000
gel filtration
43783
1 * 43783, calculation from sequence
44000
45000
active-site mutant S255A of pro-Tk-subtilisin
55000
x * 36000, activated mature codon-optimised wild-type enzyme, SDS-PAGE, x * 55000, recombinant His-tagged proform codon-optimised wild-type enzyme, SDS-PAGE
63000
gel filtration, Pro-Tk-S359C (an enzyme derivative with the mutation of the active-site serine residue to Cys)
66000
x * 66000, Pro-Tk-S359C (an enzyme derivative with the mutation of the active-site serine residue to Cys)