3.4.21.B57: pernisine
This is an abbreviated version!
For detailed information about pernisine, go to the full flat file.
Word Map on EC 3.4.21.B57
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3.4.21.B57
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hyperthermophilic
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archaeon
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subtilisin-like
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pernix
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aeropyrum
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subtilisins
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thermococcus
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prion
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medicine
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kodakaraensis
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proregion
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mesophilic
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autoprocessed
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detergents
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edta
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codon-optimised
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far-uv
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tk-subtilisin
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high-temperature
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cacl2
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n-propeptide
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roll
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hyperthermostable
- 3.4.21.B57
-
hyperthermophilic
- archaeon
-
subtilisin-like
- pernix
-
aeropyrum
- subtilisins
-
thermococcus
- prion
- medicine
- kodakaraensis
-
proregion
-
mesophilic
-
autoprocessed
- detergents
- edta
-
codon-optimised
-
far-uv
- tk-subtilisin
-
high-temperature
- cacl2
- n-propeptide
-
roll
-
hyperthermostable
Reaction
the enzyme can digest the pathological prion protein isoform (PrPSc) from different species, e.g. human, bovine, deer and mouse =
Synonyms
pernisine, subtilase, Tk-SP, Tk-subtilisin, TKS
ECTree
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Inhibitors
Inhibitors on EC 3.4.21.B57 - pernisine
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AMPHITOL 20Y-B
presence of 1% (w/v) AMPHITOL 20Y-B causes strong inhibitions, resulting in an enzyme retaining only 50% of its activity
Guanidine HCl
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5 mM, 65% loss of activity in absence of CaCl2, activation in presence of 1 mM CaCl2
Thermococcus kodakaraensis serpin
irreversibly inhibits more strongly at 80°C than at 40°C. The covalent inhibitory complex is highly stable and the ester bond between serpin and protease can be hydrolyzed only in a harsh condition, in which most proteases are denatured
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Thermococcus kodakaraensis subtilisin propeptide
potent noncompetitive inhibitor of the mature domain
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EDTA
about 20% loss of activity in the presence of 0.01% (w/v) at 80°C, about 60% loss of activity in the presence of 1% (w/v) at 80°C; activity decreases with the increasing concentration of EDTA from 0.01 to 1% (w/v)
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TPCK and TLCK, respectively chymotrypsin and trypsin-like inhibitors do not affect the activity. 1,10-phenanthroline has no effect
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additional information
the activity is retained or even enhanced in the presence of nonionic, cationic (except in the presence of SANIZOL C), and amphoteric surfactants at both 0.1 and 1% (w/v)
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