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3.4.21.B57: pernisine

This is an abbreviated version!
For detailed information about pernisine, go to the full flat file.

Word Map on EC 3.4.21.B57

Reaction

the enzyme can digest the pathological prion protein isoform (PrPSc) from different species, e.g. human, bovine, deer and mouse =

Synonyms

pernisine, subtilase, Tk-SP, Tk-subtilisin, TKS

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.21 Serine endopeptidases
                3.4.21.B57 pernisine

Inhibitors

Inhibitors on EC 3.4.21.B57 - pernisine

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INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2-mercaptoethanol
4-hydroxymercuribenzoate
-
10 mM, 80% inhibition
AMPHITOL 20Y-B
presence of 1% (w/v) AMPHITOL 20Y-B causes strong inhibitions, resulting in an enzyme retaining only 50% of its activity
Aprotinin
-
-
dithiothreitol
-
5 mM, 64% loss of activity in absence of CaCl2
DTT
42% and 52% inhibition at 1 mm and 5 mM
Guanidine HCl
-
5 mM, 65% loss of activity in absence of CaCl2, activation in presence of 1 mM CaCl2
iodoacetamide
slight inhibition
Soybean trypsin inhibitor
-
1 mg/ml, complete inhibition
-
Thermococcus kodakaraensis serpin
irreversibly inhibits more strongly at 80°C than at 40°C. The covalent inhibitory complex is highly stable and the ester bond between serpin and protease can be hydrolyzed only in a harsh condition, in which most proteases are denatured
-
Thermococcus kodakaraensis subtilisin propeptide
potent noncompetitive inhibitor of the mature domain
-
Urea
-
5 mM, 50% loss of activity in absence of CaCl2
additional information
-