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3.4.21.B57: pernisine

This is an abbreviated version!
For detailed information about pernisine, go to the full flat file.

Word Map on EC 3.4.21.B57

Reaction

the enzyme can digest the pathological prion protein isoform (PrPSc) from different species, e.g. human, bovine, deer and mouse =

Synonyms

pernisine, subtilase, Tk-SP, Tk-subtilisin, TKS

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.21 Serine endopeptidases
                3.4.21.B57 pernisine

General Stability

General Stability on EC 3.4.21.B57 - pernisine

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GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
1 mM CaCl2 stabilizes the enzyme for up to 4 h at 120°C
-
in the presence of 0.05% (w/v) nonionic surfactants, such as EMULGEN LS-114 or RHEODOL Tw-0120 V, and 0.01% (w/v) EDTA, Tk-SP retains almost its entire initial activity
in the presence of anionic surfactants, the enzyme is unstable, losing up to 80% of its activity
in the presence of anionic surfactants, Tk-SP is unstable, losing up to 80% of its activity
less stable in the presence of QUARTAMIN 60 W
stable in the presence of 0.1% (w/v) AMPHITOL 20Y-B
the activity is retained or even enhanced in the presence of nonionic, cationic (except in the presence of SANIZOL C), and amphoteric surfactants at both 0.1 and 1% (w/v)
the beta-jelly roll domain is required for hyperstabilization of the protein. This domain contains two Ca2+ ions and contributes to the stabilization of the protein only in a Ca2+-bound form
the enzyme is highly stable in the presence of 0.05% (w/v) nonionic surfactants and 0.01% (w/v) EDTA, retaining up to 80% of its activity
the enzyme is highly stable in the presence of both 0.1 and 1% (w/v) nonionic surfactants
the enzyme is stabilized in the presence of the Ca2+
the enzyme retains its activity in the presence of surfactants tested at 80°C and 90°C
the enzyme retains more than 100% of its activity in the presence of four of the nonionic surfactants, namely, EMULGEN 147, EMULGEN LS-114, EMULGEN PP-290, and RHEODOL Tw-0120 V. It is less stable in the presence of QUARTAMIN 60 W