3.4.21.B55: pyrolysin
This is an abbreviated version!
For detailed information about pyrolysin, go to the full flat file.
Word Map on EC 3.4.21.B55
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3.4.21.B55
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hyperthermophilic
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pyrococcus
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furiosus
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subtilisin-like
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subtilases
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archaeon
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hyperthermostable
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n-glycosylation
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asn
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autoproteolysis
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subtilisin
- 3.4.21.B55
-
hyperthermophilic
-
pyrococcus
- furiosus
-
subtilisin-like
-
subtilases
- archaeon
-
hyperthermostable
-
n-glycosylation
- asn
-
autoproteolysis
- subtilisin
Reaction
endopeptidase with two preferential cleavage sites in alphaS1-casein, at bonds Gln9-/-Gly10 and Leu16-/-Asn17 =
ECTree
3.4.21.B55 pyrolysinAdvanced search results
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results (Temperature Stability
Temperature Stability on EC 3.4.21.B55 - pyrolysin
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TEMPERATURE STABILITY 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
100
105
110
115
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5 min, wild-type enzyme loses about 75% of its activity, mutant enzyme D818N/D820N loses about 70% of its activity
95
additional information
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extra structural elements play a crucial role in adapting pyrolysin to hyperthermal environments
105
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20 min, wild-type enzyme loses about 45% of its activity, mutant enzyme D818N/D820N loses about 30% of its activity
110
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5 min, wild-type enzyme loses about 30% of its activity, mutant enzyme D818N/D820N loses about 20% of its activity
95
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half-life of wild-type enzyme: 12 h, half-life of mutant enzyme D818N/D820N is 18 h
95
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half-life: 12 h (wild-type enzyme), 18 h (mutant enzymes R249E and D818N/D820N), 36 h (R249E/D818N/D820N)
