3.4.21.B55: pyrolysin

This is an abbreviated version!
For detailed information about pyrolysin, go to the full flat file.

Word Map on EC 3.4.21.B55

3.4.21.B55

hyperthermophilic

pyrococcus

furiosus

subtilisin-like

subtilases

archaeon

hyperthermostable

n-glycosylation

asn

autoproteolysis

subtilisin

Reaction

endopeptidase with two preferential cleavage sites in alphaS1-casein, at bonds Gln9-/-Gly10 and Leu16-/-Asn17 =

ECTree

3 Hydrolases

3.4 Acting on peptide bonds (peptidases)

3.4.21 Serine endopeptidases

3.4.21.B55 pyrolysin

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Results	in table
1	AA Sequence
5	Cloned(Commentary)
4	General Stability
4	Inhibitors
2	kcat/KM [mM/s]
3	KM Value [mM]
5	Localization
4	Metals/Ions
5	Molecular Weight [Da]
2	Organic Solvent Stability
6	Organism
1	Oxidation Stability
4	pH Optimum
1	Posttranslational Modification
33	Protein Variants
5	Purification (Commentary)
1	Reaction
6	Reference
19	Substrates and Products (Substrate)
4	Subunits
5	Temperature Optimum [°C]
1	Temperature Range [°C]
12	Temperature Stability [°C]
3	Turnover Number [1/s]

Reference

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Reference on EC 3.4.21.B55 - pyrolysin

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721356

Dai, Z.; Fu, H.; Zhang, Y.; Zeng, J.; Tang, B.; Tang, X.F.

Insights into the maturation of hyperthermophilic pyrolysin and the roles of its N-terminal propeptide and long C-terminal extension

Appl. Environ. Microbiol.

4233-4241

2012

Pyrococcus furiosus (P72186), Pyrococcus furiosus

22504813

722300

Eggen, R.; Geerling, A.; Watts, J.; de Vos,W.M.

Characterization of pyrolysin, a hyperthermoactive serine protease from the archaebacterium Pyrococcus furiosus

FEMS Microbiol. Lett.

17-20

1990

Pyrococcus furiosus (P72186)

722600

Voorhorst, W.G.; Eggen, R.I.; Geerling, A.C.; Platteeuw, C.; Siezen, R.J.; Vos, W.M.

Isolation and characterization of the hyperthermostable serine protease, pyrolysin, and its gene from the hyperthermophilic archaeon Pyrococcus furiosus

J. Biol. Chem.

271

20426-20431

1996

Pyrococcus furiosus (P72186), Pyrococcus furiosus

8702780

723099

de Vos, W.M.; Voorhorst, W.G.; Dijkgraaf, M.; Kluskens, L.D.; van der Oost, J.; Siezen, R.J.

Purification, characterization, and molecular modeling of pyrolysin and other extracellular thermostable serine proteases from hyperthermophilic microorganisms

Methods Enzymol.

330

383-393

2001

Pyrococcus furiosus (P72186)

11210516

724041

Zeng, J.; Gao, X.; Dai, Z.; Tang, B.; Tang, X.F.

Effects of metal ions on stability and activity of hyperthermophilic pyrolysin and further stabilization of this enzyme by modification of a Ca2+-binding site

Appl. Environ. Microbiol.

2763-2772

2014

Pyrococcus furiosus

24561589

752548

Gao, X.; Zeng, J.; Yi, H.; Zhang, F.; Tang, B.; Tang, X.F.

Four inserts within the catalytic domain confer extra stability and activity to hyperthermostable pyrolysin from Pyrococcus furiosus

Appl. Environ. Microbiol.

e03228-16

2017

Pyrococcus furiosus

28003199