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3.4.21.B52: thermostable serine endopeptidase (Sulfolobus)

This is an abbreviated version!
For detailed information about thermostable serine endopeptidase (Sulfolobus), go to the full flat file.

Word Map on EC 3.4.21.B52

Reaction

The best substrate is benzyloxycarbonyl-Tyr-4-nitroanilide. Cleaves Phe-Ser-Lys-Phe-Ile-/-Ser-Phe-Asp. No activity benzyloxycarbonyl-Ile-4-nitroanilide, aminoacyl-4-nitroanilides or benzyloxycarbonyl-amino acids, oxidized insulin chain A and B, bovine pancreas ribonuclease A, bovine serum albumin or ovalbumin. =

Synonyms

proteinase I

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.21 Serine endopeptidases
                3.4.21.B52 thermostable serine endopeptidase (Sulfolobus)

Turnover Number

Turnover Number on EC 3.4.21.B52 - thermostable serine endopeptidase (Sulfolobus)

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TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
12.1
benzyloxycarbonyl-Leu-4-nitroanilide
-
pH 7.0, 80°C
23
benzyloxycarbonyl-Tyr-4-nitroanilide
-
pH 7.0, 80°C
2.2
Suc-Ala-Ala-Phe-4-nitroanilide
-
pH 7.0, 80°C
1
Suc-Ala-Ala-Pro-Phe 4-nitroanilide
-
pH 7.0, 80°C
3.3
Suc-Gly-Gly-Phe-4-nitroanilide
-
pH 7.0, 80°C
15.6
Suc-Phe-4-nitroanilide
-
pH 7.0, 80°C