3.4.21.B52: thermostable serine endopeptidase (Sulfolobus)

This is an abbreviated version!
For detailed information about thermostable serine endopeptidase (Sulfolobus), go to the full flat file.

Reaction

The best substrate is benzyloxycarbonyl-Tyr-4-nitroanilide. Cleaves Phe-Ser-Lys-Phe-Ile-/-Ser-Phe-Asp. No activity benzyloxycarbonyl-Ile-4-nitroanilide, aminoacyl-4-nitroanilides or benzyloxycarbonyl-amino acids, oxidized insulin chain A and B, bovine pancreas ribonuclease A, bovine serum albumin or ovalbumin. =

Synonyms

proteinase I

ECTree

     3 Hydrolases

         3.4 Acting on peptide bonds (peptidases)

             3.4.21 Serine endopeptidases

                3.4.21.B52 thermostable serine endopeptidase (Sulfolobus)

Advanced search results

Do not include text mining results

Include results (more...)

Include results (more...)

Results	in table
2	General Stability
9	Inhibitors
6	kcat/KM [mM/s]
6	KM Value [mM]
3	Localization
3	Metals/Ions
3	Molecular Weight [Da]
6	Organic Solvent Stability
20	Organism
3	pH Optimum
1	pI Value
1	Purification (Commentary)
1	Reaction
3	Reference
1	Specific Activity [micromol/min/mg]
11	Substrates and Products (Substrate)
1	Subunits
2	Synonyms
1	Temperature Optimum [°C]
5	Temperature Stability [°C]
6	Turnover Number [1/s]

Substrates Products

print visible entries

print all entries

Go back to the abbreviated version
of the Enzyme Summary Page.

Substrates Products on EC 3.4.21.B52 - thermostable serine endopeptidase (Sulfolobus)

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.

top print hide show all columns Go to Substrate/Product Search

Please wait a moment until the data is sorted. This message will disappear when the data is sorted.

SUBSTRATE

PRODUCT                       

REACTION DIAGRAM

ORGANISM

UNIPROT

COMMENTARY
(Substrate)

LITERATURE
(Substrate)

COMMENTARY
(Product)

LITERATURE
(Product)

Reversibility
r=reversible
ir=irreversible
?=not specified

benzyloxycarbonyl-Ala-Ala-Leu-4-nitroanilide + H2O

benzyloxycarbonyl-Ala-Ala-Leu + 4-nitroaniline

Saccharolobus solfataricus

-

1% of the activity with benzyloxycarbonyl-Tyr-4-nitroanilide

718954

-

-

?

benzyloxycarbonyl-Leu-4-nitroanilide + H2O

benzyloxycarbonyl-Leu + 4-nitroaniline

Saccharolobus solfataricus

-

36% of the activity with benzyloxycarbonyl-Tyr-4-nitroanilide

718954

-

-

?

benzyloxycarbonyl-Tyr-4-nitroanilide + H2O

benzyloxycarbonyl-Tyr + 4-nitroaniline

Saccharolobus solfataricus

-

-

718954

-

-

?

Phe-Ser-Lys-Phe-Ile-Ser-Phe-Asp + H2O

Phe-Ser-Lys-Phe-Ile + Ser-Phe-Asp

Saccharolobus solfataricus

-

no cleavage between Phe4 and Ile5

718954

-

-

?

Suc-Ala-Ala-Phe-4-nitroanilide + H2O

Suc-Ala-Ala-Phe + 4-nitroaniline

Saccharolobus solfataricus

-

3% of the activity with benzyloxycarbonyl-Tyr-4-nitroanilide

718954

-

-

?

Suc-Ala-Ala-Pro-Phe-4-nitroanilide + H2O

Suc-Ala-Ala-Pro-Phe + 4-nitroaniline

Saccharolobus solfataricus

-

2% of the activity with benzyloxycarbonyl-Tyr-4-nitroanilide

718954

-

-

?

Suc-Gly-Gly-Phe-4-nitroanilide + H2O

Suc-Gly-Gly-Phe + 4-nitroaniline

Saccharolobus solfataricus

-

6.5% of the activity with benzyloxycarbonyl-Tyr-4-nitroanilide

718954

-

-

?

Suc-Phe-4-nitroanilide + H2O

Suc-Phe + 4-nitroaniline

3 entries

additional information

?

-

Saccharolobus solfataricus

-

narrow substrate specificity. No activity with: aminoacyl-4-nitroanilides or benzyloxycarbonyl-amino acids, oxidized insulin chain A and B, bovine pancreas ribonuclease A, bovine serum albumin, ovalbumin. No activity with benzyloxycarbonyl-Ile-4-nitroanilide

718954

-

-

?

Suc-Phe-4-nitroanilide + H2O

Suc-Phe + 4-nitroaniline

Saccharolobus solfataricus

-

-

651211

-

-

?

Suc-Phe-4-nitroanilide + H2O

Suc-Phe + 4-nitroaniline

Saccharolobus solfataricus

-

18.5% of the activity with benzyloxycarbonyl-Tyr-p-nitroanilide

718954

-

-

?

Suc-Phe-4-nitroanilide + H2O

Suc-Phe + 4-nitroaniline

Saccharolobus solfataricus MT-4 / DSM 5833

-

-

651211

-

-

?